Processing and Transport of Environmental Virus Samples

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1984 Jun 1

PMID 6331312

Citations 5

Authors

D R Dahling

B A Wright

Affiliations

Soon will be listed here.

Abstract

Poliovirus-seeded tap water, conditioned with MgCl2 and passed through virus-adsorbing filters, gave better poliovirus recovery than water identically treated but conditioned with AlCl3. Elution of several filter types with beef extract yielded higher recoveries than did elution with glycine. Seeded samples filtered through various filters and stored showed considerable virus loss in 2 days when stored at 4 degrees C, whereas those stored at -70 degrees C gave stable virus recovery up to 4 days. Additionally, the use of antifoam during the elution process reduced foaming and increased virus recovery by 28%.

Citing Articles

Applicability of integrated cell culture reverse transcriptase quantitative PCR (ICC-RTqPCR) for the simultaneous detection of the four human enteric enterovirus species in disinfection studies.

Ryu H, Schrantz K, Brinkman N, Boczek L J Virol Methods. 2018; 258:35-40.

PMID: 29778790 PMC: 6662635. DOI: 10.1016/j.jviromet.2018.05.008.

Virus hazards from food, water and other contaminated environments.

Rodriguez-Lazaro D, Cook N, Ruggeri F, Sellwood J, Nasser A, Nascimento M FEMS Microbiol Rev. 2011; 36(4):786-814.

PMID: 22091646 PMC: 7114518. DOI: 10.1111/j.1574-6976.2011.00306.x.

New electropositive filter for concentrating enteroviruses and noroviruses from large volumes of water.

Karim M, Rhodes E, Brinkman N, Wymer L, Fout G Appl Environ Microbiol. 2009; 75(8):2393-9.

PMID: 19218410 PMC: 2675229. DOI: 10.1128/AEM.00922-08.

Use of bituminous coal as an alternative technique for field concentration of waterborne viruses.

Dahling D, Phirke P, Wright B, SAFFERMAN R Appl Environ Microbiol. 1985; 49(5):1222-5.

PMID: 4004235 PMC: 238533. DOI: 10.1128/aem.49.5.1222-1225.1985.

Transport of viral specimens.

Johnson F Clin Microbiol Rev. 1990; 3(2):120-31.

PMID: 2187591 PMC: 358147. DOI: 10.1128/CMR.3.2.120.

References

Farrah S, Gerba C, Wallis C, Melnick J . Concentration of viruses from large volumes of tap water using pleated membrane filters. Appl Environ Microbiol. 1976; 31(2):221-6. PMC: 169751. DOI: 10.1128/aem.31.2.221-226.1976. View

Walter R, Rudiger S . [A two-stage technique for concentration of viruses from solutions with low virus titers (e.g., drinking water)]. J Hyg Epidemiol Microbiol Immunol. 1981; 25(1):71-81. View

MORROW A . Concentration of the virus of foot and mouth disease by foam flotation. Nature. 1969; 222(5192):489-90. DOI: 10.1038/222489a0. View

Dahling D, SAFFERMAN R . Survival of enteric viruses under natural conditions in a subarctic river. Appl Environ Microbiol. 1979; 38(6):1103-10. PMC: 291253. DOI: 10.1128/aem.38.6.1103-1110.1979. View

Joret J, Block J . [Survival of enteric viruses adsorbed on glass microfibers during postal transport]. Can J Microbiol. 1981; 27(2):246-8. View

Sobsey M, Jones B . Concentration of poliovirus from tap water using positively charged microporous filters. Appl Environ Microbiol. 1979; 37(3):588-95. PMC: 243259. DOI: 10.1128/aem.37.3.588-595.1979. View

Melnick J, Safferman R, Rao V, Goyal S, Berg G, Dahling D . Round robin investigation of methods for the recovery of poliovirus from drinking water. Appl Environ Microbiol. 1984; 47(1):144-50. PMC: 239626. DOI: 10.1128/aem.47.1.144-150.1984. View

Sobsey M, Wallis C, Henderson M, Melnick J . Concentration of enteroviruses from large volumes of water. Appl Microbiol. 1973; 26(4):529-34. PMC: 379842. DOI: 10.1128/am.26.4.529-534.1973. View

Barron A, Olshevsky C, COHEN M . Characteristics of the BGM line of cells from African green monkey kidney. Brief report. Arch Gesamte Virusforsch. 1970; 32(4):389-92. DOI: 10.1007/BF01250067. View

10.

Dahling D, Berg G, Berman D . BGM, a continuous cell line more sensitive than primary rhesus and African green kidney cells for the recovery of viruses from water. Health Lab Sci. 1974; 11(4):275-82. View

11.

Payment P . Isolation of viruses from drinking water at the Point-Viau water treatment plant. Can J Microbiol. 1981; 27(4):417-20. DOI: 10.1139/m81-063. View

12.

KATZENELSON E, Fattal B, Hostovesky T . Organic flocculation: an efficient second-step concentration method for the detection of viruses in tap water. Appl Environ Microbiol. 1976; 32(4):638-9. PMC: 170320. DOI: 10.1128/aem.32.4.638-639.1976. View