The Histochemistry of Complex Carbohydrates in Certain Organs of Homozygous Brachymorphic (bm/bm) Mice

Overview

Journal Histochem J

Specialty Biochemistry

Date 1984 Jun 1

PMID 6330002

Citations 1

Authors

K Yamada

S Shimizu

K S Brown

K Kimata

Affiliations

Soon will be listed here.

Abstract

Homozygous brachymorphic (bm/bm) mice are characterized by disproportionately short stature and by undersulphated proteochondroitin sulphate in cartilage with a defect in the synthesis of the sulphate donor (3'-phosphoadenosine-5'-phosphosulphate: PAPS). In adult mice of this mutant strain, several organs have been examined by light microscopical methods of complex carbohydrate histochemistry, in comparison with those from control (C57BL/6J) mice of comparable ages. The organs included the trachea, aorta, skin, stomach, colon and cornea. In the mutant mice, connective tissue elements and components of epithelial tissues exhibited apparently weaker positive reactions for ester sulphate groupings of complex carbohydrates, as compared with those in the control mice. From these results, it is concluded that the undersulphation in complex carbohydrates is widespread throughout the connective and epithelial tissues of the mutant mice.

Citing Articles

Localization of anionic constituents in mast cell granules of brachymorphic (bm/bm) mice by using avidin-conjugated colloidal gold.

Hammel I, Shoichetman T, Amihai D, Galli S, Skutelsky E Cell Tissue Res. 2010; 339(3):561-70.

PMID: 20127366 PMC: 3645895. DOI: 10.1007/s00441-009-0919-2.

References

Sugahara K, Schwartz N . Defect in 3'-phosphoadenosine 5'-phosphosulfate synthesis in brachymorphic mice. I. Characterization of the defect. Arch Biochem Biophys. 1982; 214(2):589-601. DOI: 10.1016/0003-9861(82)90064-9. View

Yamada K . The effect of digestion with Streptomyces hyaluronidase upon certain histochemical reactions of hyaluronic acid-containing tissues. J Histochem Cytochem. 1973; 21(9):794-803. DOI: 10.1177/21.9.794. View

Yamada K, Hirano K . The histochemistry of hyaluronic acid-containing mucosubstances. J Histochem Cytochem. 1973; 21(5):469-72. DOI: 10.1177/21.5.469. View

Greene R, Brown K, Pratt R . Autoradiographic analysis of altered glycosaminoglycan synthesis in the epiphyseal cartilage of neonatal brachymorphic mice. Anat Rec. 1978; 191(1):19-29. DOI: 10.1002/ar.1091910103. View

Yamada K . The effect of digestion with chondroitinases upon certain histochemical reactions of mucosaccharide-containing tissues. J Histochem Cytochem. 1974; 22(4):266-75. DOI: 10.1177/22.4.266. View

SPICER S . DIAMINE METHODS FOR DIFFERENTIALING MUCOSUBSTANCES HISTOCHEMICALLY. J Histochem Cytochem. 1965; 13:211-34. DOI: 10.1177/13.3.211. View

Lev R, SPICER S . SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH. J Histochem Cytochem. 1964; 12:309. DOI: 10.1177/12.4.309. View

LEPPI T . Morphochemical analysis of mucous cells in the skin and slime glands of hagfishes. Histochemie. 1968; 15(1):68-78. DOI: 10.1007/BF00304575. View

LEPPI T, STOWARD P . ON THE USE OF TESTICULAR HYALURONIDASE FOR IDENTIFYING ACID MUCINS IN TISSUE SECTIONS. J Histochem Cytochem. 1965; 13:406-7. DOI: 10.1177/13.5.406. View

10.

Schwartz N, Ostrowski V, Brown K, Pratt R . Defective PAPS-synthesis in epiphyseal cartilage from brachymorphic mice. Biochem Biophys Res Commun. 1978; 82(1):173-8. DOI: 10.1016/0006-291x(78)90592-2. View

11.

Barrnett R, Seligman A . Histochemical demonstration of protein-bound alpha-acylamido carboxyl groups. J Biophys Biochem Cytol. 1958; 4(2):169-76. PMC: 2224342. DOI: 10.1083/jcb.4.2.169. View

12.

Sugahara K, Schwartz N . Defect in 3'-phosphoadenosine 5'-phosphosulfate synthesis in brachymorphic mice. II. Tissue distribution of the defect. Arch Biochem Biophys. 1982; 214(2):602-9. DOI: 10.1016/0003-9861(82)90065-0. View

13.

Yamada K . Concanavalin A-perioxidase-diaminobenzidine (Con-A-PO-DAB)-alcian blue (AB): a reliable method for dual staining of complex carbohydrates. Histochemistry. 1976; 47(2):159-69. DOI: 10.1007/BF00492563. View

14.

Sugahara K, Schwartz N . Defect in 3'-phosphoadenosine 5'-phosphosulfate formation in brachymorphic mice. Proc Natl Acad Sci U S A. 1979; 76(12):6615-8. PMC: 411917. DOI: 10.1073/pnas.76.12.6615. View

15.

Orkin R, Pratt R, Martin G . Undersulfated chondroitin sulfate in the cartilage matrix of brachymorphic mice. Dev Biol. 1976; 50(1):82-94. DOI: 10.1016/0012-1606(76)90069-5. View

16.

Lane P, DICKIE M . Three recessive mutations producing disproportionate dwarfing in mice: achondroplasia, brachymorphic, and stubby. J Hered. 1968; 59(5):300-8. DOI: 10.1093/oxfordjournals.jhered.a107725. View

17.

Orkin R, Williams B, Cranley R, Poppke D, Brown K . Defects in the cartilaginous growth plates of brachymorphic mice. J Cell Biol. 1977; 73(2):287-99. PMC: 2109918. DOI: 10.1083/jcb.73.2.287. View

18.

Pennypacker J, Kimata K, Brown K . Brachymorphic mice (bm/bm): a generalized biochemical defect expressed primarily cartilage. Dev Biol. 1981; 81(2):280-7. DOI: 10.1016/0012-1606(81)90291-8. View