Concanavalin A-perioxidase-diaminobenzidine (Con-A-PO-DAB)-alcian Blue (AB): a Reliable Method for Dual Staining of Complex Carbohydrates

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1976 May 28

PMID 60314

Citations 18

Authors

K Yamada

Affiliations

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Abstract

A method has been established for the dual staining of complex carbohydrates in light microscopy. It is a combined concanavalin A-peroxidase-diaminobenzidine (Con-A-PO-DAB)-alcian blue (AB) (pH 2.5) method, and with this method it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues and acidic groupings of complex carbohydrates in tissues brown and blue respectively. Histochemical experiments using histological sections with reactive complex carbohydrates and casein films containing carbohydrates of known chemical structure have substantiated the validity of the above significance of the dual staining. Thus, the present dual staining method is a reliable one and a new addition to a series of dual staining techniques hitherto employed in the light microscopic histochemistry of complex carbohydrates.

Citing Articles

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Ultrastructural visualization of concanavalin A binding sites using alkaline phosphatase-labeled Con A and ultrathin glycol methacrylate sections.

Yamada K, Shimizu S Histochemistry. 1980; 67(1):31-41.

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The histochemistry of complex carbohydrates in the scrotum of the boar.

Tsukise A, Yamada K Histochemistry. 1981; 72(4):511-21.

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Histochemical demonstration of asparagine-linked oligosaccharides in glycoproteins of human placenta and umbilical cord tissues by means of almond glycopeptidase digestion.

Yamada K, Shimizu S, Takahashi N Histochem J. 1983; 15(12):1239-50.

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Histochemistry of complex carbohydrates in the hairy skin of the domestic pig.

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