Nucleotide Sequence of Avian Carcinoma Virus MH2: Two Potential Onc Genes, One Related to Avian Virus MC29 and the Other Related to Murine Sarcoma Virus 3611

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 May 1

PMID 6328485

Citations 25

Authors

N C Kan

C S Flordellis

G E Mark

P H Duesberg

T S Papas

Affiliations

Soon will be listed here.

Abstract

The 5.2-kilobase (kb) RNA genome of avian carcinoma virus MH2 has the genetic structure 5'-delta gag (0.2 kb)- mht (1.2 kb)-myc (1.4 kb)-c (0.4 kb)-poly(A) (0.2 kb)-3'. delta gag is a partial retroviral core protein gene, mht and myc are cell-derived MH2-specific sequences, and c is the 3'-terminal retroviral vector sequence. Here we have determined the nucleotide sequence of 3.5 kb from the 3' end of delta gag to the 3' end of molecularly cloned proviral MH2 DNA, in order to elucidate the genetic structure of the virus and to compare it with other mht - and myc-containing oncogenic viruses as well as with the chicken proto-myc gene. The following results were obtained: (i) delta gag- mht forms a hybrid gene with a contiguous reading frame of 2682 nucleotides that terminates with a stop codon near the 3' end of mht . The 3' 969 nucleotides of mht up to the stop codon are 80% sequence related to the onc-specific raf sequence of murine sarcoma virus 3611 (94% homologous at the deduced amino acid level). (ii) The myc sequence is preceded by an RNA splice acceptor site shared with the cellular proto-myc gene, beyond which it is colinear up to a 3'-termination codon and 40 noncoding nucleotides with the myc sequences of avian retrovirus MC29 and chicken proto-myc. Thus, myc forms, together with a 5' retroviral exon, a second MH2-specific gene. (iii) myc is followed by the 3'-terminal c region of about 400 nucleotides, which is colinear with that of Rous sarcoma virus except for a substitution near the 5' end of the long terminal repeat. It is concluded that MH2 contains two genes with oncogenic potential, the delta gag- mht gene, which is closely related to the delta gag-raf transforming gene of MSV 3611, and the myc gene, which is related to the transforming gene of MC29. Furthermore, it may be concluded that the cellular proto-onc genes, which on sequence transduction become viral onc genes, are a small group because among the 19 known onc sequences, 5 are shared by different taxonomic groups of viruses of which the mht /raf homology is the closest determined so far.

Citing Articles

Host range restrictions of oncogenes: myc genes transform avian but not mammalian cells and mht/raf genes transform mammalian but not avian cells.

Li R, Zhou R, Duesberg P Proc Natl Acad Sci U S A. 1996; 93(15):7522-7.

PMID: 8755507 PMC: 38778. DOI: 10.1073/pnas.93.15.7522.

Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62.

Gupta S, Seth A, Davis R Proc Natl Acad Sci U S A. 1993; 90(8):3216-20.

PMID: 8386367 PMC: 46270. DOI: 10.1073/pnas.90.8.3216.

Characterization of a myc-containing retrovirus generated by propagation of an MH2 viral subgenomic RNA.

MARTIN P, Henry C, Ferre F, Bechade C, Begue A, Calothy C J Virol. 1986; 57(3):1191-4.

PMID: 3951018 PMC: 252859. DOI: 10.1128/JVI.57.3.1191-1194.1986.

Mutagenesis of avian carcinoma virus MH2: only one of two potential transforming genes (delta gag-myc) transforms fibroblasts.

Zhou R, Kan N, Papas T, Duesberg P Proc Natl Acad Sci U S A. 1985; 82(19):6389-93.

PMID: 3876558 PMC: 390721. DOI: 10.1073/pnas.82.19.6389.

Activation of human raf transforming genes by deletion of normal amino-terminal coding sequences.

Stanton Jr V, Cooper G Mol Cell Biol. 1987; 7(3):1171-9.

PMID: 3561413 PMC: 365190. DOI: 10.1128/mcb.7.3.1171-1179.1987.

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