A New Avian Erythroblastosis Virus, AEV-H, Carries ErbB Gene Responsible for the Induction of Both Erythroblastosis and Sarcomas

The genome structure of a newly isolated avian erythroblastosis virus, AEV-H, was analyzed. Using DNA probes specific for the LTR sequence of SR-RSV-A, and for the erbA gene and the erbB gene of the ES4 strain of AEV, we have shown that the genome of AEV-H is 35S in size and carries the erbB gene but not the erbA gene. Comparison of the restriction sites of molecularly cloned AEV-H DNA with that of cloned DNA of the associated virus revealed that the env gene of the associated virus was replaced with the erbB gene to generate AEV-H. The genome structure of AEV-H is, therefore, determined to be 5'-gag-pol-erbB-3'. Moreover, we have isolated a mutant of AEV-H, td-130, that can induce sarcomas but not erythroblastosis in chickens. The restriction analysis of proviral DNA of the td-130 showed that it carries a deletion of about 150 to 200 nucleotides in the erbB gene. These data indicate that the erbB protein is responsible for both erythroblastosis and sarcomas.