On the Origin of Chromosomal Aberrations in Human Peripheral Lymphocytes in Vitro. I. Experiments with Neurospora Endonuclease and Polyethylene Glycol

Overview

Journal Hum Genet

Specialty Genetics

Date 1984 Jan 1

PMID 6327498

Citations 2

Authors

C Nowak

G Obe

Affiliations

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Abstract

Post-treatment of mutagen-treated human peripheral lymphocytes with a single-strand specific endonuclease from Neurospora crassa leads to a significant elevation of the rate of structural chromosomal aberrations. Our results indicate that DNA double-strand breaks (DSB) are ultimate lesions for the formation of chromosomal aberrations in the G1 and G2 phase of the cell cycle and probably also in the S-phase. Post-treatment of X-irradiated G2 cells with polyethylene glycol (PEG) leads to an elevation of the frequencies of chromatid type aberrations. This result is taken as an indication that nucleases from PEG-damaged lysosomes transform lesions in X-ray damaged chromosomes to DSB. With respect to the origin of chromosomal aberrations, our results are in favour of the breakage and reunion hypothesis of K. Sax , and not of Revell 's exchange hypothesis.

Citing Articles

Association between Genomic Instability and Evolutionary Chromosomal Rearrangements in Neotropical Primates.

Puntieri F, Andrioli N, Nieves M Genome Biol Evol. 2018; 10(7):1647-1656.

PMID: 29905781 PMC: 6366543. DOI: 10.1093/gbe/evy119.

Non random distribution of lesions induced by deoxyribonuclease I in human chromosomes.

Nuzzo F, Casati A, Raimondi E Cytotechnology. 2012; 1(1):19-24.

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