Cytosolic Ca2+-dependent Neutral Proteinases from Rabbit Liver: Activation of the Proenzymes by Ca2+ and Substrate

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Jan 1

PMID 6320172

Citations 12

Authors

S Pontremoli

E Melloni

F Salamino

B Sparatore

M Michetti

B L HORECKER

Affiliations

Soon will be listed here.

Abstract

Two neutral Ca2+-dependent proteinases, differing in molecular size, have been isolated from rabbit liver. Both are recovered as inactive proenzymes that can be converted to the active forms by high (0.1-1.0 mM) concentrations of Ca2+ in the absence of substrate or, in the presence of a protein substrate, by low (1-5 microM) concentrations of Ca2+. The activated proteinases required only 1-5 microM Ca2+ for maximal activity. Substrates hydrolyzed were denatured globin, globin, casein, and to a lesser extent, several extracellular proteins; no digestion was observed with several intracellular cytosolic enzymes tested. Only those proteins that served as substrates were capable of promoting conversion of the proenzymes to the active low-Ca2+-requiring proteinases.

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