Limited Autolysis of Ca2+-activated Neutral Protease (CANP) Changes Its Sensitivity to Ca2+ Ions

Overview

Journal J Biochem

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Jul 1

PMID 6270080

Citations 22

Authors

K Suzuki

S Tsuji

S Kubota

Y Kimura

K Imahori

Affiliations

Soon will be listed here.

Abstract

Ca2+-activated neutral protease (CANP) usually requires mM Ca2+ for activation. The sensitivity of CANP to Ca2+ is greatly enhanced by passing it through a casein-Sepharose column in the presence of Ca2+ ions. This conversion is ascribed to autolysis of CANP. The converted enzyme required 40 microM Ca2+ for 50% activation. Various properties of the converted enzyme were very similar to those of CANP-I, recently found in canine heart muscle. Names of "m-CANP" and "mu-CANP" are proposed for CANPs which require mM and microM order Ca2+ for inactivation, respectively.

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