Importance of Oxidative Metabolism in T Cell Cytotoxicity: a Comparison of Cloned T Cells and Spleen Cells

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1983 Dec 1

PMID 6317542

Citations 1

Authors

K J THORNE

D Franks

Affiliations

Soon will be listed here.

Abstract

To distinguish between direct and indirect involvement of oxygen metabolites in CTL cytotoxicity a comparison was made of cloned CTLs and mixed cells from mouse spleen. Tumour cells could be protected from cloned CTLs and from spleen CTLs by the thiol protecting reducing agents DTT and DETC. Cytotoxic activity was inhibited by diversion of reducing power from NAD(P)H to artificial electron acceptors and by the inhibitor of NAD(P) linked enzymes cibacron blue. Although H2O2 formation could be detected during the lysis of P815 by spleen CTLs it did not prove to be a necessary requirement for cytolysis since it was not formed when glutaraldehyde-treated P815 cells were lysed. Of the scavengers of toxic oxygen metabolites tested only the hydroxyl radical scavenger sodium benzoate inhibited cytotoxicity.

Citing Articles

Hydroxyl radical scavengers inhibit human lectin-dependent cellular cytotoxicity.

Melinn M, McLaughlin H Immunology. 1986; 58(2):197-202.

PMID: 3011654 PMC: 1452669.

References

Berke G, Gabison D . Energy requirements of the binding and lytic steps of T lymphocyte-mediated cytolysis of leukemic cells in vitro. Eur J Immunol. 1975; 5(10):671-5. DOI: 10.1002/eji.1830051004. View

BRUNNER K, Mauel J, Cerottini J, Chapuis B . Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968; 14(2):181-96. PMC: 1409286. View

GRANGER G, KOLB W . Lymphocyte in vitro cytotoxicity: mechanisms of immune and non-immune small lymphocyte mediated target L cell destruction. J Immunol. 1968; 101(1):111-20. View

Leroux A, Kaplan J . Presence of red cell type NADH-methemoglobin reductase (NADH-diaphorase) in human non erythroid cells. Biochem Biophys Res Commun. 1972; 49(4):945-50. DOI: 10.1016/0006-291x(72)90303-8. View

Berke G, Amos D . Mechanism of lymphocyte-mediated cytolysis. The LMC cycle and its role in transplantation immunity. Transplant Rev. 1973; 17(0):71-107. DOI: 10.1111/j.1600-065x.1973.tb00124.x. View

Martz E . Mechanism of specific tumor-cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp Top Immunobiol. 1977; 7:301-61. DOI: 10.1007/978-1-4684-3054-7_9. View

MacDonald H, Koch C . Energy metabolism and T-cell-mediated cytolysis. I. Synergism between inhibitors of respiration and glycolysis. J Exp Med. 1977; 146(3):698-709. PMC: 2180805. DOI: 10.1084/jem.146.3.698. View

Sanderson C, GLAUERT A . The mechanism of T cell mediated cytotoxicity. V. Morphological studies by electron microscopy. Proc R Soc Lond B Biol Sci. 1977; 198(1132):315-323. DOI: 10.1098/rspb.1977.0100. View

Robinson J, Briggs R, Karnovsky M . Localization of D-amino acid oxidase on the cell surface of human polymorphonuclear leukocytes. J Cell Biol. 1978; 77(1):59-71. PMC: 2110022. DOI: 10.1083/jcb.77.1.59. View

10.

Sanderson C, GLAUERT A . The mechanism of T-cell mediated cytotoxicity. VI. T-cell projections and their role in target cell killing. Immunology. 1979; 36(1):119-29. PMC: 1457384. View

11.

Grimm E, Price Z, Bonavida B . Studies on the induction and expression of T cell-mediated immunity. VIII. Effector-target junctions and target cell membrane disruption during cytolysis. Cell Immunol. 1979; 46(1):77-99. DOI: 10.1016/0008-8749(79)90247-8. View

12.

Repine J, Eaton J, Anders M, Hoidal J, Fox R . Generation of hydroxyl radical by enzymes, chemicals, and human phagocytes in vitro. Detection with the anti-inflammatory agent, dimethyl sulfoxide. J Clin Invest. 1979; 64(6):1642-51. PMC: 371318. DOI: 10.1172/JCI109626. View

13.

THORNE K, Svvennsen R, Franks D . Role of hydrogen peroxide in the cytotoxic reaction of T lymphocytes. Clin Exp Immunol. 1980; 39(2):486-95. PMC: 1538068. View

14.

Edwards Y, Potter J, Hopkinson D . Human FAD-dependent NAD(P)H diaphorase. Biochem J. 1980; 187(2):429-36. PMC: 1161809. DOI: 10.1042/bj1870429. View

15.

MacDonald H, Cerottini J, Ryser J, Maryanski J, Taswell C, Widmer M . Quantitation and cloning of cytolytic T lymphocytes and their precursors. Immunol Rev. 1980; 51:93-123. DOI: 10.1111/j.1600-065x.1980.tb00318.x. View

16.

Uyttenhove C, Van Snick J, Boon T . Immunogenic variants obtained by mutagenesis of mouse mastocytoma P815. I. Rejection by syngeneic mice. J Exp Med. 1980; 152(5):1175-83. PMC: 2185985. DOI: 10.1084/jem.152.5.1175. View

17.

Ngo T, Lenhoff H . A sensitive and versatile chromogenic assay for peroxidase and peroxidase-coupled reactions. Anal Biochem. 1980; 105(2):389-97. DOI: 10.1016/0003-2697(80)90475-3. View

18.

Sanderson C . The mechanism of T-cell mediated cytotoxicity. VIII. Zeiosis corresponds to irreversible phase (programming for lysis) in steps leading to lysis. Immunology. 1981; 42(2):201-6. PMC: 1458082. View

19.

Light D, Walsh C, OCallaghan A, Goetzl E, Tauber A . Characteristics of the cofactor requirements for the superoxide-generating NADPH oxidase of human polymorphonuclear leukocytes. Biochemistry. 1981; 20(6):1468-76. DOI: 10.1021/bi00509a010. View

20.

Devlin R, Lin C, Perper R, Dougherty H . Evaluation of free radical scavengers in studies of lymphocyte-mediated cytolysis. Immunopharmacology. 1981; 3(2):147-59. DOI: 10.1016/0162-3109(81)90016-3. View