Role of Hydrogen Peroxide in the Cytotoxic Reaction of T Lymphocytes

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1980 Feb 1

PMID 6248285

Citations 8

Authors

K J THORNE

R J Svvennsen

D Franks

Affiliations

Soon will be listed here.

Abstract

Evidence is presented that T lymphocyte cytotoxicity is mediated by hydrogen peroxide (H2O2). At a concentration of 5 x 10(-4) M H2O2 induced 51Cr release from pre-labelled P815 mastocytoma cells. H2O2 was generated when T lymphocytes from mouse spleen were exposed to P815 cells. The concentration of H2O produced was apparently one thousand times lower than the concentration required to lyse the P815 cells. This suggests that the H2O2 is produced and acts at a highly localized site on the target cell. Sulphydryl groups on the target cell were particularly sensitive both to H2O2 and to spleen cell attack. The activity of the spleen cells was inhibited by cyanide and azide and by reducing agents which protected the target cells. Cytotoxicity was enhanced by agents which prevented H2O2 breakdown.

Citing Articles

Does each Component of Reactive Oxygen Species have a Dual Role in the Tumor Microenvironment?.

Hao S, Cai D, Gou S, Li Y, Liu L, Tang X Curr Med Chem. 2023; 31(31):4958-4986.

PMID: 37469162 PMC: 11340293. DOI: 10.2174/0929867331666230719142202.

Nanomedicine strategies for treatment of secondary spinal cord injury.

White-Schenk D, Shi R, Leary J Int J Nanomedicine. 2015; 10:923-38.

PMID: 25673988 PMC: 4321603. DOI: 10.2147/IJN.S75686.

Forkhead box protein A1 inhibits the expression of uncoupling protein 2 in hydrogen peroxide-induced A549 cell line.

Song L, Xu Z, Li L, Hu M, Cheng L, Chen L Cell Stress Chaperones. 2013; 19(1):53-60.

PMID: 23625627 PMC: 3857427. DOI: 10.1007/s12192-013-0433-z.

Role of sulphydryl groups in T lymphocyte-mediated cytotoxicity.

THORNE K, Free J, Franks D Clin Exp Immunol. 1982; 50(3):644-50.

PMID: 6984671 PMC: 1536835.

In vivo damage of rat lungs by oxygen metabolites.

Johnson K, Fantone 3rd J, Kaplan J, Ward P J Clin Invest. 1981; 67(4):983-93.

PMID: 6894154 PMC: 370656. DOI: 10.1172/jci110149.

References

BRUNNER K, Mauel J, Cerottini J, Chapuis B . Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs. Immunology. 1968; 14(2):181-96. PMC: 1409286. View

Guilbault G, Brignac Jr P, Juneau M . New substrates for the fluorometric determination of oxidative enzymes. Anal Chem. 1968; 40(8):1256-63. DOI: 10.1021/ac60264a027. View

Babior B, Kipnes R, Curnutte J . Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest. 1973; 52(3):741-4. PMC: 302313. DOI: 10.1172/JCI107236. View

Klebanoff S . Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes. Semin Hematol. 1975; 12(2):117-42. View

Clark R, Klebanoff S, Einstein A, Fefer A . Peroxidase-H2O2-halide system: Cytotoxic effect on mammalian tumor cells. Blood. 1975; 45(2):161-70. View

Clark R, Klebanoff S . Neutrophil-mediated tumor cell cytotoxicity: role of the peroxidase system. J Exp Med. 1975; 141(6):1442-7. PMC: 2189847. DOI: 10.1084/jem.141.6.1442. View

Heikkila R, Cabbat F, Cohen G . In vivo inhibition of superoxide dismutase in mice by diethyldithiocarbamate. J Biol Chem. 1976; 251(7):2182-5. View

Golstein P, Smith E . Mechanism of T-cell-mediated cytolysis: the lethal hit stage. Contemp Top Immunobiol. 1977; 7:273-300. DOI: 10.1007/978-1-4684-3054-7_8. View

Martz E . Mechanism of specific tumor-cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp Top Immunobiol. 1977; 7:301-61. DOI: 10.1007/978-1-4684-3054-7_9. View

10.

Sanderson C, GLAUERT A . The mechanism of T cell mediated cytotoxicity. V. Morphological studies by electron microscopy. Proc R Soc Lond B Biol Sci. 1977; 198(1132):315-323. DOI: 10.1098/rspb.1977.0100. View

11.

THORNE K, Svvennsen R, Franks D . Role of hydrogen peroxide and peroxidase in the cytotoxicity of Trypanosoma dionisii by human granulocytes. Infect Immun. 1978; 21(3):798-805. PMC: 422068. DOI: 10.1128/iai.21.3.798-805.1978. View

12.

Nathan C, Silverstein S, Brukner L, COHN Z . Extracellular cytolysis by activated macrophages and granulocytes. II. Hydrogen peroxide as a mediator of cytotoxicity. J Exp Med. 1979; 149(1):100-13. PMC: 2184732. DOI: 10.1084/jem.149.1.100. View

13.

Rosen H, Klebanoff S . Bactericidal activity of a superoxide anion-generating system. A model for the polymorphonuclear leukocyte. J Exp Med. 1979; 149(1):27-39. PMC: 2184741. DOI: 10.1084/jem.149.1.27. View

14.

Sanderson C, GLAUERT A . The mechanism of T-cell mediated cytotoxicity. VI. T-cell projections and their role in target cell killing. Immunology. 1979; 36(1):119-29. PMC: 1457384. View

15.

Matter A . Microcinematographic and electron microscopic analysis of target cell lysis induced by cytotoxic T lymphocytes. Immunology. 1979; 36(2):179-90. PMC: 1457473. View

16.

Dunn T, Potter M . A transplantable mast-cell neoplasm in the mouse. J Natl Cancer Inst. 1957; 18(4):587-601. View