Inhibition by Purines of the Inotropic Action of Isoprenaline in Rat Atria

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1983 Sep 1

PMID 6317127

Citations 9

Authors

P R Hughes

T W Stone

Affiliations

Soon will be listed here.

Abstract

The effects of a series of adenosine derivatives were examined on the catecholamine-stimulated electrically-driven rat left atrium in vitro. All the purines tested reduced the positive inotropic action of isoprenaline, 0.1 microM, with the potency order: L-N6-phenlylisopropyladenosine (L-PIA) greater than 5'-N-ethylcarboxamide adenosine (NECA) greater than D-PIA greater than 2-chloroadenosine greater than adenosine. Dipyridamole did not change the IC50 of adenosine. The adenosine deaminase inhibitor, 2'deoxycoformycin, produced a small but nonsignificant shift to the left of the adenosine concentration-response curve. The cardiac depressant effects of these purines were reversed by theophylline and the IC50 values were unchanged in the presence of atropine or in atria taken from reserpine-treated rats. It is concluded that the purine receptor mediating these effects should not be classified on the A1/A2 system. The relationship between functionally characterized purine receptors and those originally defined as modulating adenylate cyclase is discussed.

Citing Articles

Evidence for adenosine receptor-mediated isoprenaline-antagonistic effects of the adenosine analogs PIA and NECA on force of contraction in guinea-pig atrial and ventricular cardiac preparations.

Bohm M, Bruckner R, Meyer W, Nose M, Schmitz W, Scholz H Naunyn Schmiedebergs Arch Pharmacol. 1985; 331(2-3):131-9.

PMID: 3003587 DOI: 10.1007/BF00634229.

On the type of receptor involved in the inhibitory action of adenosine at the neuromuscular junction.

Ribeiro J, Sebastiao A Br J Pharmacol. 1985; 84(4):911-8.

PMID: 2988684 PMC: 1987060. DOI: 10.1111/j.1476-5381.1985.tb17385.x.

Demonstration of Ri-type adenosine receptors in bovine myocardium by radioligand binding.

Lohse M, Ukena D, Schwabe U Naunyn Schmiedebergs Arch Pharmacol. 1985; 328(3):310-6.

PMID: 2984586 DOI: 10.1007/BF00515559.

Pronounced cholinergic but only moderate purinergic effects in isolated atrial and ventricular heart muscle from cats.

Kemmer M, Jakob H, Nawrath H Br J Pharmacol. 1989; 97(4):1191-8.

PMID: 2790381 PMC: 1854613. DOI: 10.1111/j.1476-5381.1989.tb12578.x.

Effects of 1,3-dipropyl-8-cyclopentylxanthine (DPCPX), a highly selective adenosine receptor antagonist, on force of contraction in guinea-pig atrial and ventricular cardiac preparations.

von der Leyen H, Schmitz W, Scholz H, Scholz J, Lohse M, Schwabe U Naunyn Schmiedebergs Arch Pharmacol. 1989; 340(2):204-9.

PMID: 2554151 DOI: 10.1007/BF00168970.

References

DEGUBAREFF T, SLEATOR Jr W . EFFECTS OF CAFFEINE ON MAMMALIAN ATRIAL MUSCLE, AND ITS INTERACTION WITH ADENOSINE AND CALCIUM. J Pharmacol Exp Ther. 1965; 148:202-14. View

Collis M . Evidence for an A1-adenosine receptor in the guinea-pig atrium. Br J Pharmacol. 1983; 78(1):207-12. PMC: 2044793. DOI: 10.1111/j.1476-5381.1983.tb09381.x. View

Burnstock G . Review lecture. Neurotransmitters and trophic factors in the autonomic nervous system. J Physiol. 1981; 313:1-35. PMC: 1274434. DOI: 10.1113/jphysiol.1981.sp013648. View

Agarwal R . Inhibitors of adenosine deaminase. Pharmacol Ther. 1982; 17(3):399-429. DOI: 10.1016/0163-7258(82)90023-7. View

Hawthorn M, Broadley K . Evidence from use of neuronal uptake inhibition that beta 1-adrenoceptors, but not beta 2-adrenoceptors, are innervated. J Pharm Pharmacol. 1982; 34(10):664-6. DOI: 10.1111/j.2042-7158.1982.tb04697.x. View

Stone T . Cell-membrane receptors for purines. Review. Biosci Rep. 1982; 2(2):77-90. DOI: 10.1007/BF01116173. View

Schrader J, Baumann G, Gerlach E . Adenosine as inhibitor of myocardial effects of catecholamines. Pflugers Arch. 1977; 372(1):29-35. DOI: 10.1007/BF00582203. View

WALDECK B . Some effects of caffeine and aminophylline on the turnover of catecholamines in the brain. J Pharm Pharmacol. 1971; 23(11):824-30. DOI: 10.1111/j.2042-7158.1971.tb10198.x. View

Londos C, Cooper D, Wolff J . Subclasses of external adenosine receptors. Proc Natl Acad Sci U S A. 1980; 77(5):2551-4. PMC: 349439. DOI: 10.1073/pnas.77.5.2551. View

10.

Schrader J, Nees S, Gerlach E . Evidence for a cell surface adenosine receptor on coronary myocytes and atrial muscle cells. Studies with an adenosine derivative of high molecular weight. Pflugers Arch. 1977; 369(3):251-7. DOI: 10.1007/BF00582192. View

11.

Smellie F, Daly J, Dunwiddie T, Hoffer B . The dextro and levorotatory isomers of N-phenylisopropyladenosine: stereospecific effects on cyclic AMP-formation and evoked synaptic responses in brain slices. Life Sci. 1979; 25(20):1739-48. DOI: 10.1016/0024-3205(79)90477-6. View

12.

Bruns R . Adenosine receptor activation in human fibroblasts: nucleoside agonists and antagonists. Can J Physiol Pharmacol. 1980; 58(6):673-91. DOI: 10.1139/y80-110. View

13.

Berkowitz B, Tarver J, Spector S . Release of norepinephrine in the central nervous system by theophylline and caffeine. Eur J Pharmacol. 1970; 10(1):64-71. DOI: 10.1016/0014-2999(70)90158-5. View

14.

Ewald D . Potentiation of postjunctional cholinergic sensitivity of rat diaphragm muscle by high-energy-phosphate adenine nucleotides. J Membr Biol. 1976; 29(1-2):47-65. DOI: 10.1007/BF01868951. View

15.

Kuroda Y . Physiological roles of adenosine derivatives which are released during neurotransmission in mammalian brain. J Physiol (Paris). 1978; 74(5):463-70. View

16.

Schutz W, TUISL E . Evidence against adenylate cyclase-coupled adenosine receptors in the guinea pig heart. Eur J Pharmacol. 1981; 76(2-3):285-8. DOI: 10.1016/0014-2999(81)90516-1. View

17.

van Calker D, Muller M, Hamprecht B . Adenosine regulates via two different types of receptors, the accumulation of cyclic AMP in cultured brain cells. J Neurochem. 1979; 33(5):999-1005. DOI: 10.1111/j.1471-4159.1979.tb05236.x. View

18.

Hedqvist P, Fredholm B . Inhibitory effect of adenosine on adrenergic neuroeffector transmission in the rabbit heart. Acta Physiol Scand. 1979; 105(1):120-2. DOI: 10.1111/j.1748-1716.1979.tb06321.x. View

19.

Dunwiddie T, Hoffer B . Adenine nucleotides and synaptic transmission in the in vitro rat hippocampus. Br J Pharmacol. 1980; 69(1):59-68. PMC: 2044173. DOI: 10.1111/j.1476-5381.1980.tb10883.x. View

20.

Hopkins S, Goldie R . A species difference in the uptake of adenosine by heart. Biochem Pharmacol. 1971; 20(12):3359-65. DOI: 10.1016/0006-2952(71)90440-0. View