Immunological Phenotype of Lymphomas Induced by Avian Leukosis Viruses

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1983 Jun 1

PMID 6308424

Citations 5

Authors

L C Chen

S A Courtneidge

J M Bishop

Affiliations

Soon will be listed here.

Abstract

The production of immunoglobulin by six cell lines derived from bursal tumors induced by avian leukosis virus follows two general patterns: (i) three cell lines that have been extensively passaged in culture synthesize and secrete light chains only; (ii) three cell lines that are recently isolated produce and secrete monomeric immunoglobulin M in addition to free light chains. All six cell lines synthesize and secrete both glycosylated and unglycosylated forms of light chain. We conclude that the cell lines established from lymphomas induced by avian leukosis virus represent relatively mature, but possibly abnormal, stages in the development of chicken B-lymphocytes. The immunoglobulin M produced by the cell lines failed to form detectable immune complexes with avian leukosis virus. It therefore appears that the immunoglobulin M is not directed against viral antigens and that autogenous antigenic stimulus cannot account for the sustained growth of the neoplastic B-lymphocytes.

Citing Articles

Isolation of monoclonal antibodies specific for products of avian oncogene myb.

Evan G, LEWIS G, Bishop J Mol Cell Biol. 1984; 4(12):2843-50.

PMID: 6084811 PMC: 369296. DOI: 10.1128/mcb.4.12.2843-2850.1984.

Identification of a provirally activated c-Ha-ras oncogene in an avian nephroblastoma via a novel procedure: cDNA cloning of a chimaeric viral-host transcript.

Westaway D, Papkoff J, MOSCOVICI C, Varmus H EMBO J. 1986; 5(2):301-9.

PMID: 3011401 PMC: 1166733. DOI: 10.1002/j.1460-2075.1986.tb04213.x.

Expression of v-rel induces mature B-cell lines that reflect the diversity of avian immunoglobulin heavy- and light-chain rearrangements.

Barth C, Humphries E Mol Cell Biol. 1988; 8(12):5358-68.

PMID: 2854197 PMC: 365638. DOI: 10.1128/mcb.8.12.5358-5368.1988.

Lability of leukosis virus enhancer-binding proteins in avian hematopoeitic cells.

Ruddell A, Linial M, Schubach W, Groudine M J Virol. 1988; 62(8):2728-35.

PMID: 2839698 PMC: 253706. DOI: 10.1128/JVI.62.8.2728-2735.1988.

Rearrangements of chicken immunoglobulin genes in lymphoid cells transformed by the avian retroviral oncogene v-rel.

Chen L, Lim M, Bose Jr H, Bishop J Proc Natl Acad Sci U S A. 1988; 85(2):549-53.

PMID: 2829193 PMC: 279588. DOI: 10.1073/pnas.85.2.549.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Melchers F . Biosynthesis of the carbohydrate portion of immunoglobulins. Kinetics of synthesis and secretion of [3H] leucine-, [3H] galactose- and [3H] mannose-labelled myeloma protein by two plasma-cell tumours. Biochem J. 1970; 119(4):765-72. PMC: 1179464. DOI: 10.1042/bj1190765. View

Grant J, Sanders B, Hood L . Partial amino acid sequences of chicken and turkey immunoglobulin light chains. Homology with mammalian lambda chains. Biochemistry. 1971; 10(16):3123-32. DOI: 10.1021/bi00792a022. View

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

Cooper M, Purchase H, Bockman D, Gathings W . Studies on the nature of the abnormality of B cell differentiation in avian lymphoid leukosis: production of heterogeneous IgM by tumor cells. J Immunol. 1974; 113(4):1210-22. View

Hihara H, Shimizu T, Yamamoto H . Establishment of tumor cell lines cultured from chickens with avian lymphoid leukosis. Natl Inst Anim Health Q (Tokyo). 1974; 14(4):163-73. View

Purchase H, Gilmour D . Lymphoid leukosis in chickens chemically bursectomized and subsequently inoculated with bursa cells. J Natl Cancer Inst. 1975; 55(4):851-5. DOI: 10.1093/jnci/55.4.851. View

Vitetta E, UHR J . Immunoglobulin-receptors revisited. Science. 1975; 189(4207):964-9. DOI: 10.1126/science.1083069. View

Cleveland D, Fischer S, Kirschner M, Laemmli U . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977; 252(3):1102-6. View

10.

McGrath M, Witte O, Pincus T, Weissman I . Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity. J Virol. 1978; 25(3):923-7. PMC: 525988. DOI: 10.1128/JVI.25.3.923-927.1978. View

11.

Levinson A, Oppermann H, LEVINTOW L, Varmus H, Bishop J . Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein. Cell. 1978; 15(2):561-72. DOI: 10.1016/0092-8674(78)90024-7. View

12.

Oppermann H, Levinson A, Varmus H, LEVINTOW L, Bishop J . Uninfected vertebrate cells contain a protein that is closely related to the product of the avian sarcoma virus transforming gene (src). Proc Natl Acad Sci U S A. 1979; 76(4):1804-8. PMC: 383480. DOI: 10.1073/pnas.76.4.1804. View

13.

Siden E, Baltimore D, Clark D, Rosenberg N . Immunoglobulin synthesis by lymphoid cells transformed in vitro by Abelson murine leukemia virus. Cell. 1979; 16(2):389-96. DOI: 10.1016/0092-8674(79)90014-x. View

14.

McGrath M, Weissman I . AKR leukemogenesis: identification and biological significance of thymic lymphoma receptors for AKR retroviruses. Cell. 1979; 17(1):65-75. DOI: 10.1016/0092-8674(79)90295-2. View

15.

Tartakoff A, Vassalli P . Plasma cell immunoglobulin M molecules. Their biosynthesis, assembly, and intracellular transport. J Cell Biol. 1979; 83(2 Pt 1):284-99. PMC: 2111544. DOI: 10.1083/jcb.83.2.284. View

16.

Vassalli P, Tedghi R, Tartakoff A, Jaton J . Evidence for hydrophobic region within heavy chains of mouse B lymphocyte membrane-bound IgM. Proc Natl Acad Sci U S A. 1979; 76(11):5515-9. PMC: 411679. DOI: 10.1073/pnas.76.11.5515. View

17.

Neiman P, Payne L, Weiss R . Viral DNA in bursal lymphomas induced by avian leukosis viruses. J Virol. 1980; 34(1):178-86. PMC: 288684. DOI: 10.1128/JVI.34.1.178-186.1980. View

18.

GAZZOLO L, Samarut J, Bouabdelli M, Blanchet J . Early precursors in the erythroid lineage are the specific target cells of avian erythroblastosis virus in vitro. Cell. 1980; 22(3):683-91. DOI: 10.1016/0092-8674(80)90544-9. View

19.

Lee J, Ihle J . Chronic immune stimulation is required for Moloney leukaemia virus-induced lymphomas. Nature. 1981; 289(5796):407-9. DOI: 10.1038/289407a0. View

20.

Payne G, Courtneidge S, Crittenden L, Fadly A, Bishop J, Varmus H . Analysis of avian leukosis virus DNA and RNA in bursal tumours: viral gene expression is not required for maintenance of the tumor state. Cell. 1981; 23(2):311-22. DOI: 10.1016/0092-8674(81)90127-6. View