Retrovirus Purification: Method That Conserves Envelope Glycoprotein and Maximizes Infectivity

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1978 Mar 1

PMID 205680

Citations 21

Authors

M McGrath

O Witte

T Pincus

I L Weissman

Affiliations

Soon will be listed here.

Abstract

A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.

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The Susceptibilities of Respiratory Syncytial Virus to Nucleolin Receptor Blocking and Antibody Neutralization are Dependent upon the Method of Virus Purification.

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Tagging retrovirus vectors with a metal binding peptide and one-step purification by immobilized metal affinity chromatography.

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Three isoforms of cyclophilin A associated with human immunodeficiency virus type 1 were found by proteomics by using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry.

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