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Genetic Analysis of a Pleiotropic Deletion Mutation (delta Igf) in Bacillus Subtilis

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1983 May 1
PMID 6302085
Citations 15
Authors
Y Fujita
T Fujita
Affiliations
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Abstract

A delta igf mutation of Bacillus subtilis (formerly called fdpAl) is a large deletion causing pleiotropic defects. The mapping of the delta igf deletion by phage PBS1 transduction revealed the following map order: sacA, thiC, hsrE, delta igf, ts199, purA. To analyze the pleiotropic nature of the delta igf mutation, mutants affected in each property of the pleiotropic mutation were isolated, and the mutations were mapped. iol and gnt mutants could not grow on inositol and gluconate, respectively, and fdp mutants were affected only in fructose-bisphosphatase. The map order from sacA to purA was as follows: sacA, thiC, hsrE, iol-6, gnt-4, fdp-74, hsrB, ts199, purA. The delta igf deletion covered loci from iol-6 to hsrB.

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