Identification and Nucleotide Sequence of the Promoter Region of the Bacillus Subtilis Gluconate Operon

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Feb 11

PMID 2419835

Citations 21

Authors

Y Fujita

T Fujita

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence (742 bp) of the promoter region of the Bacillus subtilis gluconate (gnt) operon is presented. Nuclease Sl mapping revealed the start point of the transcription and suggested that the expression of this operon is probably regulated at the transcriptional level. The sequences of the -35 and -10 regions suggested that RNA polymerase possessing sigma-43 may recognize this structure. The 223 bp fragment containing 100 bp upstream from the transcription start site actually exhibited a promoter activity when cloned in a promoter probe vector of pPL603B. This promoter activity was highly derepressed and although still under catabolite repression. The fragment on a high copy plasmid could titrate a regulator of the gnt operon so that the expression of the operon on the host chromosome also became derepressed.

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References

Setlow P . Inability of detect cyclic AMP in vegetative or sporulating cells or dormant spores of Bacillus megaterium. Biochem Biophys Res Commun. 1973; 52(2):365-72. DOI: 10.1016/0006-291x(73)90720-1. View

Gitt M, Wang L, Doi R . A strong sequence homology exists between the major RNA polymerase sigma factors of Bacillus subtilis and Escherichia coli. J Biol Chem. 1985; 260(12):7178-85. View

Tinoco Jr I, Borer P, Dengler B, Levin M, Uhlenbeck O, Crothers D . Improved estimation of secondary structure in ribonucleic acids. Nat New Biol. 1973; 246(150):40-1. DOI: 10.1038/newbio246040a0. View

Dickson R, Abelson J, Barnes W, Reznikoff W . Genetic regulation: the Lac control region. Science. 1975; 187(4171):27-35. DOI: 10.1126/science.1088926. View

Shaw W . Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975; 43:737-55. DOI: 10.1016/0076-6879(75)43141-x. View

Pastan I, Adhya S . Cyclic adenosine 5'-monophosphate in Escherichia coli. Bacteriol Rev. 1976; 40(3):527-51. PMC: 413971. DOI: 10.1128/br.40.3.527-551.1976. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

Gryczan T, Contente S, Dubnau D . Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J Bacteriol. 1978; 134(1):318-29. PMC: 222249. DOI: 10.1128/jb.134.1.318-329.1978. View

Sadaie Y, Burtis K, Doi R . Purification and characterization of a kanamycin nucleotidyltransferase from plasmid pUB110-carrying cells of Bacillus subtilis. J Bacteriol. 1980; 141(3):1178-82. PMC: 293806. DOI: 10.1128/jb.141.3.1178-1182.1980. View

10.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

11.

Fujita Y, Freese E . Isolation and properties of a Bacillus subtilis mutant unable to produce fructose-bisphosphatase. J Bacteriol. 1981; 145(2):760-7. PMC: 217176. DOI: 10.1128/jb.145.2.760-767.1981. View

12.

Williams D, Duvall E, Lovett P . Cloning restriction fragments that promote expression of a gene in Bacillus subtilis. J Bacteriol. 1981; 146(3):1162-5. PMC: 216974. DOI: 10.1128/jb.146.3.1162-1165.1981. View

13.

Goldfarb D, Doi R, Rodriguez R . Expression of Tn9-derived chloramphenicol resistance in Bacillus subtilis. Nature. 1981; 293(5830):309-11. DOI: 10.1038/293309a0. View

14.

Aiba H, Adhya S, de Crombrugghe B . Evidence for two functional gal promoters in intact Escherichia coli cells. J Biol Chem. 1981; 256(22):11905-10. View

15.

Kroyer J, Chang S . The promoter-proximal region of the Bacillus licheniformis penicillinase gene: Nucleotide sequence and predicted leader peptide sequence. Gene. 1981; 15(4):343-7. DOI: 10.1016/0378-1119(81)90177-3. View

16.

Moran Jr C, Lang N, LeGrice S, Lee G, Stephens M, Sonenshein A . Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982; 186(3):339-46. DOI: 10.1007/BF00729452. View

17.

Fujita Y, Fujita T . Genetic analysis of a pleiotropic deletion mutation (delta igf) in Bacillus subtilis. J Bacteriol. 1983; 154(2):864-9. PMC: 217540. DOI: 10.1128/jb.154.2.864-869.1983. View

18.

Banner C, Moran Jr C, Losick R . Deletion analysis of a complex promoter for a developmentally regulated gene from Bacillus subtilis. J Mol Biol. 1983; 168(2):351-65. DOI: 10.1016/s0022-2836(83)80023-0. View

19.

Nihashi J, Fujita Y . Catabolite repression of inositol dehydrogenase and gluconate kinase syntheses in Bacillus subtilis. Biochim Biophys Acta. 1984; 798(1):88-95. DOI: 10.1016/0304-4165(84)90014-x. View

20.

Shimotsu H, Henner D . Characterization of the Bacillus subtilis tryptophan promoter region. Proc Natl Acad Sci U S A. 1984; 81(20):6315-9. PMC: 391914. DOI: 10.1073/pnas.81.20.6315. View