Protons Block the Dark Current of Isolated Retinal Rods

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Apr 1

PMID 6300877

Citations 7

Authors

P Mueller

E N Pugh Jr

Affiliations

Soon will be listed here.

Abstract

Membrane currents of isolated frog rods were recorded with the suction pipette technique and tested by perfusion techniques for their sensitivity to H+. The following facts have been established. (i) Increased [H+] suppresses the Na+ conductance of the outer segment rapidly and reversibly. (ii) H+ acts in the rod interior. (iii) The [H+] necessary to cause a 50% decrement in Na+ conductance is inversely related to the [Ca2+] over 5 orders of magnitude. (iv) The sensitivity to H+ and the sensitivity to light, as a function of [Ca2+], have the same slope. Thus, H+ act like light in effecting membrane current suppression but behave as if their effect is mediated through Ca2+. Based on these results and properties of rod disk membrane phosphodiesterase, we propose that protons produced in the light-activated hydrolysis of cGMP liberate Ca2+ from the disks by ion exchange.

Citing Articles

Protons suppress the dark current of frog retinal rods.

Liebman P, Mueller P, Pugh Jr E J Physiol. 1984; 347:85-110.

PMID: 6608584 PMC: 1199436. DOI: 10.1113/jphysiol.1984.sp015055.

Rod light response augmented by active phosphodiesterase.

Shimoda Y, Hurley J, Miller W Proc Natl Acad Sci U S A. 1984; 81(2):616-9.

PMID: 6320200 PMC: 344730. DOI: 10.1073/pnas.81.2.616.

Incorporation of ion channels from bovine rod outer segments into planar lipid bilayers.

Hanke W, Kaupp U Biophys J. 1984; 46(5):587-95.

PMID: 6093905 PMC: 1435059. DOI: 10.1016/S0006-3495(84)84057-6.

Docosahexaenoate-containing phospholipids in sarcoplasmic reticulum and retinal photoreceptors. A proposal for a role in Ca2+-ATPase calcium transport.

Infante J Mol Cell Biochem. 1987; 74(2):111-6.

PMID: 2955214 DOI: 10.1007/BF00224948.

Light-induced losses and dark recovery rates of guanosine 3',5'-cyclic monophosphate in rod outer segments of intact amphibian photoreceptors.

Cohen A, Blazynski C J Gen Physiol. 1988; 92(6):731-46.

PMID: 2851028 PMC: 2228925. DOI: 10.1085/jgp.92.6.731.

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