Transcription of Mouse Mammary Tumor Virus: Identification of a Candidate MRNA for the Long Terminal Repeat Gene Product

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1983 Apr 1

PMID 6298469

Citations 25

Authors

D A Wheeler

J S Butel

D Medina

R D Cardiff

G L Hager

Affiliations

Soon will be listed here.

Abstract

We have examined an assortment of preneoplastic and neoplastic mouse mammary tissues for the presence of an mRNA which could encode the putative long terminal repeat gene product of mouse mammary tumor virus. We report here the detection of a novel mouse mammary tumor virus-specific, polyadenylic acid-containing transcript in certain preneoplastic and neoplastic mammary tissue of BALB/c mice. The molecule is 1.6 kilobases in length and contains sequences from the transcriptional leader and the U3 region of the proviral DNA. The upstream terminus of the 3' information lies 75 to 80 nucleotides from the beginning of the long terminal repeat open reading frame, in close proximity to a consensus splice acceptor in the DNA. The transcript was detected in hormonally or chemically induced neoplastic, preneoplastic, and lactating mammary tissue of BALB/c mice, but not in preneoplastic or tumor tissue induced by exogenous viruses in any strain of mice examined. This implies that the RNA we observed is transcribed from an endogenous provirus template.

Citing Articles

Convergent capture of retroviral superantigens by mammalian herpesviruses.

Aswad A, Katzourakis A Nat Commun. 2015; 6:8299.

PMID: 26400439 PMC: 4667437. DOI: 10.1038/ncomms9299.

C3H mouse mammary tumor virus superantigen function requires a splice donor site in the envelope gene.

Mustafa F, Lozano M, Dudley J J Virol. 2000; 74(20):9431-40.

PMID: 11000212 PMC: 112372. DOI: 10.1128/jvi.74.20.9431-9440.2000.

Expression of mouse mammary tumor virus superantigen mRNA in the thymus correlates with kinetics of self-reactive T-cell loss.

Barnett A, Mustafa F, Wrona T, Lozano M, Dudley J J Virol. 1999; 73(8):6634-45.

PMID: 10400761 PMC: 112748. DOI: 10.1128/JVI.73.8.6634-6645.1999.

Negative-acting factor and superantigen are separable activities of the mouse mammary tumor virus long terminal repeat.

Wintersperger S, Salmons B, Miethke T, Erfle V, Wagner H, Gunzburg W Proc Natl Acad Sci U S A. 1995; 92(7):2745-9.

PMID: 7708717 PMC: 42295. DOI: 10.1073/pnas.92.7.2745.

Increased concentration of an indigenous proviral mouse mammary tumor virus long terminal repeat-containing transcript is associated with neoplastic transformation of mammary epithelium in C3H/Sm mice.

Graham D, Medina D, Smith G J Virol. 1984; 49(3):819-27.

PMID: 6321767 PMC: 255543. DOI: 10.1128/JVI.49.3.819-827.1984.

References

Medina D, DeOME K . Influence of mammary tumor virus on the tumor-producing capabilities of nodule outgrowth free of mammary tumor virus. J Natl Cancer Inst. 1968; 40(6):1303-8. View

Schlom J, Michalides R, Kufe D, Hehlmann R, Spiegelman S, Bentvelzen P . A comparative study of the biologic and molecular basis of murine mammary carcinoma: a model for human breast cancer. J Natl Cancer Inst. 1973; 51(2):541-51. View

Jamjoom G, Naso R, Arlinghaus R . Further characterization of intracellular precursor polyproteins of Rauscher leukemia virus. Virology. 1977; 78(1):11-34. DOI: 10.1016/0042-6822(77)90075-7. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Deeley R, Gordon J, Burns A, Mullinix K, Binastein M, Goldberg R . Primary activation of the vitellogenin gene in the rooster. J Biol Chem. 1977; 252(22):8310-9. View

McMaster G, Carmichael G . Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977; 74(11):4835-8. PMC: 432050. DOI: 10.1073/pnas.74.11.4835. View

Weiss S, Varmus H, Bishop J . The size and genetic composition of virus-specific RNAs in the cytoplasm of cells producing avian sarcoma-leukosis viruses. Cell. 1977; 12(4):983-92. DOI: 10.1016/0092-8674(77)90163-5. View

Berk A, Sharp P . Spliced early mRNAs of simian virus 40. Proc Natl Acad Sci U S A. 1978; 75(3):1274-8. PMC: 411453. DOI: 10.1073/pnas.75.3.1274. View

Rothenberg E, Donoghue D, Baltimore D . Analysis of a 5' leader sequence on murine leukemia virus 21S RNA: heteroduplex mapping with long reverse transcriptase products. Cell. 1978; 13(3):435-51. DOI: 10.1016/0092-8674(78)90318-5. View

10.

McGrath C, Marineau E, Voyles B . Changes in MuMTV DNA and RNA levels in Balb/c mammary epithelial cells during malignant transformation by exogenous MuTV and by hormones. Virology. 1978; 87(2):339-53. DOI: 10.1016/0042-6822(78)90139-3. View

11.

McGrath C, Jones R . Hormonal induction of mammary tumor viruses and its implications for carcinogenesis. Cancer Res. 1978; 38(11 Pt 2):4112-25. View

12.

Pauley R, Medina D, Socher S . Murine mammary tumor virus expression during mammary tumorigenesis in BALB/c mice. J Virol. 1979; 29(2):483-93. PMC: 353180. DOI: 10.1128/JVI.29.2.483-493.1979. View

13.

Cohen J, Varmus H . Endogenous mammary tumour virus DNA varies among wild mice and segregates during inbreeding. Nature. 1979; 278(5703):418-23. DOI: 10.1038/278418a0. View

14.

Sen G, Smith S, Marcus S, Sarkar N . Identification of the messenger RNAs coding for the gag and env gene products of the murine mammary tumor virus. Proc Natl Acad Sci U S A. 1979; 76(4):1736-40. PMC: 383465. DOI: 10.1073/pnas.76.4.1736. View

15.

Reeder R . The nucleotide sequence of the initiation and termination sites for ribosomal RNA transcription in X. laevis. Cell. 1979; 18(2):485-99. DOI: 10.1016/0092-8674(79)90066-7. View

16.

Michalides R, van Deemter L, Nusse R, Hageman P . Induction of mouse mammary tumor virus RNA in mammary tumors of BALB/c mice treated with urethane, X-irradiation, and hormones. J Virol. 1979; 31(1):63-72. PMC: 353422. DOI: 10.1128/JVI.31.1.63-72.1979. View

17.

Cohen J, Majors J, Varmus H . Organization of mouse mammary tumor virus-specific DNA endogenous to BALB/c mice. J Virol. 1979; 32(2):483-96. PMC: 353580. DOI: 10.1128/JVI.32.2.483-496.1979. View

18.

Anderson S, Naso R, Davis J, Bowen J . Polyprotein precursors to mouse mammary tumor virus proteins. J Virol. 1979; 32(2):507-16. PMC: 353582. DOI: 10.1128/JVI.32.2.507-516.1979. View

19.

Medina D, Butel J, Socher S . Mouse mammary tumor virus genome expression in chemical carcinogen-induced mammary tumors in low- and high-tumor-incidence mouse strains. Proc Natl Acad Sci U S A. 1979; 76(10):5360-4. PMC: 413142. DOI: 10.1073/pnas.76.10.5360. View

20.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View