Nucleotide Sequence of Satellite DNA Contained in the Eliminated Genome of Ascaris Lumbricoides

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Dec 11

PMID 6296780

Citations 25

Authors

F Muller

P Walker

P Aeby

H Neuhaus

H FELDER

E Back

H Tobler

Affiliations

Soon will be listed here.

Abstract

Several restriction endonuclease fragments isolated from highly repetitive satellite DNA of the chromatin eliminating nematode Ascaris lumbricoides var. suum have been cloned. Each type of restriction fragment corresponds to a different variant of the same related ancestral sequence. These variants differ by small deletions, insertions and single base substitutions. Restriction and DBM blot analyses show that members of the same variant class are tandemly linked and therefore are physically separated from other variant classes. A comparison of all the determined sequences establishes a 121 bp long and AT rich consensus sequence. There is evidence for an internal short range periodicity of 11 bp length, indicating that the Ascaris satellite initially may have evolved from an ancestral undecamer sequence. The satellite DNA sequences are mostly but not entirely eliminated from the presumptive somatic cells during chromatin diminution. We have no evidence for transcriptional activity of satellite DNA at any stage or tissue analyzed.

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References

Miklos G, Gill A . Nucleotide sequences of highly repeated DNAs; compilation and comments. Genet Res. 1982; 39(1):1-30. DOI: 10.1017/s0016672300020711. View

Streeck R, Moritz K, Beer K . Chromatin diminution in Ascaris suum: nucleotide sequence of the eliminated satellite DNA. Nucleic Acids Res. 1982; 10(11):3495-502. PMC: 320726. DOI: 10.1093/nar/10.11.3495. View

Singer M . Highly repeated sequences in mammalian genomes. Int Rev Cytol. 1982; 76:67-112. DOI: 10.1016/s0074-7696(08)61789-1. View

Gall J, Stephenson E, Erba H, Diaz M . Histone genes are located at the sphere loci of newt lampbrush chromosomes. Chromosoma. 1981; 84(2):159-71. DOI: 10.1007/BF00399128. View

Gillespie D, Spiegelman S . A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965; 12(3):829-42. DOI: 10.1016/s0022-2836(65)80331-x. View

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

Tobler H, Smith K, Ursprung H . Molecular aspects of chromatin elimination in Ascaris lumbricoides. Dev Biol. 1972; 27(2):190-203. DOI: 10.1016/0012-1606(72)90097-8. View

Oudet P, Chambon P . Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973; 36(1):32-8. DOI: 10.1111/j.1432-1033.1973.tb02881.x. View

Beams H, Kessel R . The problem of germ cell determinants. Int Rev Cytol. 1974; 39:413-79. DOI: 10.1016/s0074-7696(08)60944-4. View

10.

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

11.

Moritz K, Roth G . Complexity of germline and somatic DNA in Ascaris. Nature. 1976; 259(5538):55-7. DOI: 10.1038/259055a0. View

12.

Laskey R, Mills A . Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977; 82(2):314-6. DOI: 10.1016/0014-5793(77)80609-1. View

13.

McMaster G, Carmichael G . Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977; 74(11):4835-8. PMC: 432050. DOI: 10.1073/pnas.74.11.4835. View

14.

Alwine J, Kemp D, Stark G . Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977; 74(12):5350-4. PMC: 431715. DOI: 10.1073/pnas.74.12.5350. View

15.

Clarkson S, Kurer V, Smith H . Sequence organization of a cloned tDNA met fragment from Xenopus laevis. Cell. 1978; 14(3):713-24. DOI: 10.1016/0092-8674(78)90253-2. View

16.

Goldstein P, Straus N . Molecular characterization of Ascaris suum DNA and of chromatin diminution. Exp Cell Res. 1978; 116(2):462-6. DOI: 10.1016/0014-4827(78)90471-8. View

17.

Kuhn O, Tobler H . Quantitative analysis of RNA, glycogen and nucleotides from different developmental stages of Ascaris lumbricoides (var. suum). Biochim Biophys Acta. 1978; 521(1):251-66. DOI: 10.1016/0005-2787(78)90268-x. View

18.

Roop D, Nordstrom J, Tsai S, Tsai M, OMalley B . Transcription of structural and intervening sequences in the ovalbumin gene and identification of potential ovalbumin mRNA precursors. Cell. 1978; 15(2):671-85. DOI: 10.1016/0092-8674(78)90035-1. View

19.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

20.

Wieslander L . A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal Biochem. 1979; 98(2):305-9. DOI: 10.1016/0003-2697(79)90145-3. View