Gene Amplification in Methotrexate-resistant Mouse Cells. IV. Different DNA Sequences Are Amplified in Different Resistant Lines

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Nov 11

PMID 6294610

Citations 13

Authors

R Caizzi

C J Bostock

Affiliations

Soon will be listed here.

Abstract

DNA was purified from double minutes isolated from MTX-resistant EL4/8 mouse lymphoma cells, digested to completion with Bam H1 restriction endonuclease and cloned in lambda-1059. The properties of the library suggest that the DNA from which it was made was not detectably contaminated with non-dm chromosome material, and that the library is essentially complete for sequences contained in Bam H1 restriction fragments between 9 and 19 kb. The inserts of some selected lambda-recombinants were subcloned in pBR328 or pAT153 to separate sequences of differing repetition frequency. Clones representative of different classes of sequences were used as probes to Southern transfers of Bam H1 digested total nuclear DNAs of various MTX-resistant cell lines. The results clearly show that the amplified unit of each cell line has a unique structure, and that different amplified units differ widely in their sequence composition.

Citing Articles

Gene amplification in methotrexate-resistant mouse cells. V. Intact amplified units can be transferred to and amplified in methotrexate-sensitive mouse L cells.

Bostock C, Clark E Chromosoma. 1983; 88(1):31-41.

PMID: 6884154 DOI: 10.1007/BF00329501.

Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.

Ardeshir F, Giulotto E, Zieg J, Brison O, Liao W, Stark G Mol Cell Biol. 1983; 3(11):2076-88.

PMID: 6656763 PMC: 370074. DOI: 10.1128/mcb.3.11.2076-2088.1983.

Increased dihydrofolate reductase, double minutes and increased nucleolar activation in methotrexate-resistant HeLa cells.

Delinassios J, Talieri M Experientia. 1983; 39(12):1394-5.

PMID: 6653732 DOI: 10.1007/BF01990122.

Amplification and expression of human alpha-globin genes in Chinese hamster ovary cells.

Lau Y, Lin C, Kan Y Mol Cell Biol. 1984; 4(8):1469-75.

PMID: 6493227 PMC: 368936. DOI: 10.1128/mcb.4.8.1469-1475.1984.

Co-amplification of double minute chromosomes, multiple drug resistance, and cell surface P-glycoprotein in DNA-mediated transformants of mouse cells.

Robertson S, Ling V, Stanners C Mol Cell Biol. 1984; 4(3):500-6.

PMID: 6144041 PMC: 368728. DOI: 10.1128/mcb.4.3.500-506.1984.

References

COURTENAY V, ROBINS A . Loss of resistance to methotrexate in L5178Y mouse leukemia grown in vitro. J Natl Cancer Inst. 1972; 49(1):45-53. View

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Biedler J, Spengler B . Metaphase chromosome anomaly: association with drug resistance and cell-specific products. Science. 1976; 191(4223):185-7. DOI: 10.1126/science.942798. View

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

Levan A, Levan G, Mitelman F . Chromosomes and cancer. Hereditas. 1977; 86(1):15-30. DOI: 10.1111/j.1601-5223.1977.tb01208.x. View

Alt F, Kellems R, Bertino J, Schimke R . Selective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. J Biol Chem. 1978; 253(5):1357-70. View

Nunberg J, Kaufman R, Schimke R, Urlaub G, Chasin L . Amplified dihydrofolate reductase genes are localized to a homogeneously staining region of a single chromosome in a methotrexate-resistant Chinese hamster ovary cell line. Proc Natl Acad Sci U S A. 1978; 75(11):5553-6. PMC: 393004. DOI: 10.1073/pnas.75.11.5553. View

Kellems R, Morhenn V, Pfendt E, Alt F, Schimke R . Polyoma virus and cyclic AMP-mediated control of dihydrofolate reductase mRNA abundance in methotrexate-resistant mouse fibroblasts. J Biol Chem. 1979; 254(2):309-18. View

10.

Dolnick B, Berenson R, Bertino J, Kaufman R, Nunberg J, Schimke R . Correlation of dihydrofolate reductase elevation with gene amplification in a homogeneously staining chromosomal region in L5178Y cells. J Cell Biol. 1979; 83(2 Pt 1):394-402. PMC: 2111538. DOI: 10.1083/jcb.83.2.394. View

11.

Bostock C, Clark E, Harding N, Mounts P, Tyler-Smith C, Van Heyningen V . The development of resistance to methotrexate in a mouse melanoma cell line. I. Characterisation of the dihydrofolate reductases and chromosomes in sensitive and resistant cells. Chromosoma. 1979; 74(2):153-77. DOI: 10.1007/BF00292270. View

12.

Kaufman R, BROWN P, Schimke R . Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes. Proc Natl Acad Sci U S A. 1979; 76(11):5669-73. PMC: 411711. DOI: 10.1073/pnas.76.11.5669. View

13.

Bostock C, Clark E . Satellite DNA in large marker chromosomes of methotrexate-resistant mouse cells. Cell. 1980; 19(3):709-15. DOI: 10.1016/s0092-8674(80)80047-x. View

14.

Morrison D . Transformation and preservation of competent bacterial cells by freezing. Methods Enzymol. 1979; 68:326-31. DOI: 10.1016/0076-6879(79)68023-0. View

15.

Melera P, Lewis J, Biedler J, Hession C . Antifolate-resistant Chinese hamster cells. Evidence for dihydrofolate reductase gene amplification among independently derived sublines overproducing different dihydrofolate reductases. J Biol Chem. 1980; 255(14):7024-8. View

16.

Karn J, Brenner S, Barnett L, Cesareni G . Novel bacteriophage lambda cloning vector. Proc Natl Acad Sci U S A. 1980; 77(9):5172-6. PMC: 350019. DOI: 10.1073/pnas.77.9.5172. View

17.

Sillar R, Young B . A new method for the preparation of metaphase chromosomes for flow analysis. J Histochem Cytochem. 1981; 29(1):74-8. DOI: 10.1177/29.1.6162882. View

18.

Scalenghe F, Turco E, Edstrom J, Pirrotta V, Melli M . Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma. 1981; 82(2):205-16. DOI: 10.1007/BF00286105. View

19.

GEORGE D, Powers V . Cloning of DNA from double minutes of Y1 mouse adrenocortical tumor cells: evidence for gene amplification. Cell. 1981; 24(1):117-23. DOI: 10.1016/0092-8674(81)90507-9. View

20.

Ish-Horowicz D, Burke J . Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981; 9(13):2989-98. PMC: 327326. DOI: 10.1093/nar/9.13.2989. View