Alternative-substrate Inhibition and the Kinetic Mechanism of the Beta-galactoside/proton Symport of Escherichia Coli

Overview

Journal Biochem J

Specialty Biochemistry

Date 1982 Jun 15

PMID 6289801

Citations 4

Authors

M G Page

Y H Jou

Affiliations

Soon will be listed here.

Abstract

The effects of competing alternative substrates on the rate of uptake by galactoside/proton symport were investigated. These experiments produced a decrease in apparent maximum velocity with increased alternative-substrate concentration that cannot be accounted for by a simple ordered mechanism. This, together with non-linearities in the variation of the apparent kinetic constants with alternative-substrate concentration, can be accounted for by a random mechanism for galactoside and proton binding.

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