Quantitative Analysis of Proton-linked Transport System. Beta-Galactoside Exit in Escherichia Coli

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 May 15

PMID 6249273

Citations 6

Authors

I R Booth

W A Hamilton

Affiliations

Soon will be listed here.

Abstract

The exit of lactose and thiomethyl-beta-D-galactoside from Escherichia coli ML308-225 has been studied to determine the role of carrier-dependent (zero-trans efflux) and carrier-independent (leak) processes. On the basis of its sensitivity to p-chloromercuribenzene sulphonate the exit of lactose was found to be almost wholly mediated by the carrier. Consistent with this conclusion was the finding that the rate of exit of this sugar was dependent on the external pH, being considerably slower at acid pH. On the other hand exit of thiomethyl-beta-D-galactoside was found to be composed of both carrier-dependent and carrier-independent processes. Both processes exhibited first-order kinetics with the rate constants for zero-trans efflux and leak being 0.137 min-1 and 0.079 min-1, respectively. The relevance of these findings for out earlier proposal for the methods of attenuation of solute accumulation is discussed [Booth, Mitchell & Hamilton (1979) Biochem. J. 182, 687--696].

Citing Articles

Branched-Chain Amino Acid Transport in Cytoplasmic Membranes of Leuconostoc mesenteroides subsp. dextranicum CNRZ 1273.

Winters D, Poolman B, Hemme D, Konings W Appl Environ Microbiol. 1991; 57(11):3350-4.

PMID: 16348591 PMC: 183970. DOI: 10.1128/aem.57.11.3350-3354.1991.

Alternative-substrate inhibition and the kinetic mechanism of the beta-galactoside/proton symport of Escherichia coli.

Page M, Jou Y Biochem J. 1982; 204(3):681-8.

PMID: 6289801 PMC: 1158407. DOI: 10.1042/bj2040681.

The effects of partial and selective reduction in the components of the proton-motive force on lactose uptake in Escherichia coli.

Ahmed S, Booth I Biochem J. 1981; 200(3):583-9.

PMID: 6282254 PMC: 1163580. DOI: 10.1042/bj2000583.

Quantitative measurements of the proton-motive force and its relation to steady state lactose accumulation in Escherichia coli.

Ahmed S, Booth I Biochem J. 1981; 200(3):573-81.

PMID: 6282253 PMC: 1163579. DOI: 10.1042/bj2000573.

Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.

Giffard P, Rowland G, Kroll R, Stewart L, Bakker E, Booth I J Bacteriol. 1985; 164(2):904-10.

PMID: 2865250 PMC: 214337. DOI: 10.1128/jb.164.2.904-910.1985.

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