Intracellular Transport of the Transmembrane Glycoprotein G of Vesicular Stomatitis Virus Through the Golgi Apparatus As Visualized by Electron Microscope Radioautography

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1982 Jul 1

PMID 6288738

Citations 8

Authors

J J Bergeron

G J Kotwal

G Levine

P Bilan

R Rachubinski

M Hamilton

G C Shore

H P GHOSH

Affiliations

Soon will be listed here.

Abstract

The intracellular migration of G protein in vesicular stomatitis virus-infected cells was visualized by light and electron microscope radioautography after a 2-min pulse with [3H]mannose followed by nonradioactive chase for various intervals. The radioactivity initially (at 5-10 min) appeared predominantly in the endoplasmic reticulum, and the [3H]mannose-labeled G protein produced was sensitive to endoglycosidase H. Silver grains were subsequently (at 30-40 min) observed over the Golgi apparatus, and the [3H]mannose-labeled G protein became resistant to endoglycosidase H digestion. Our data directly demonstrate the intracellular transport of a plasmalemma-destined transmembrane glycoprotein through the Golgi apparatus.

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