Isolation and Characterization of Yeast Mutants Deficient in Adenylate Cyclase and CAMP-dependent Protein Kinase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Apr 1

PMID 6285379

Citations 156

Authors

K Matsumoto

I Uno

Y Oshima

T Ishikawa

Affiliations

Soon will be listed here.

Abstract

Mutants of Saccharomyces cerevisiae that require cAMP for growth have been isolated. Some of the mutants isolated were deficient in adenylate cyclase activity and mapped at a locus, cyr1, located near the centromere of chromosome X. Growth of cells carrying the cyr1 mutation was arrested at the G1 phase of the yeast cell cycle in the absence of cAMP. The cyr1 mutation was suppressed by a secondary mutation designated bcy1. The bcy1 mutation bypassed the need for cAMP for growth. The bcy1 mutants had extremely low levels of cAMP-binding protein and cAMP-dependent protein kinase but produced a high level of cAMP-independent protein kinase. The results indicate that cAMP is an essential factor for yeast cells to proceed through the cell cycle via the activation of protein kinase.

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References

Lindegren G, Hwang Y, Oshima Y, LINDEGREN C . Genetical mutants induced by ethyl methanesulfonate in Saccharomyces. Can J Genet Cytol. 1965; 7(3):491-9. DOI: 10.1139/g65-064. View

Snow R . An enrichment method for auxotrophic yeast mutants using the antibiotic 'nystatin'. Nature. 1966; 211(5045):206-7. DOI: 10.1038/211206a0. View

Sy J, Richter D . Content of cyclic 3',5'-adenosine monophosphate and adenylyl cyclase in yeast at various growth conditions. Biochemistry. 1972; 11(15):2788-91. DOI: 10.1021/bi00765a009. View

Flawia M, Torres H . Adenylate cyclase activity in Neurospora crassa. I. General properties. J Biol Chem. 1972; 247(21):6873-9. View

Uno I, Ishikawa T . Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus. J Bacteriol. 1973; 113(3):1240-8. PMC: 251689. DOI: 10.1128/jb.113.3.1240-1248.1973. View

Takai Y, Yamamura H, NISHIZUKA Y . Adenosine 3':5'-monophosphate-dependent protein kinase from yeast. J Biol Chem. 1974; 249(2):530-5. View

Hartwell L . Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974; 38(2):164-98. PMC: 413849. DOI: 10.1128/br.38.2.164-198.1974. View

Terenzi H, Flawia M, Torres H . A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity. Biochem Biophys Res Commun. 1974; 58(4):990-6. DOI: 10.1016/s0006-291x(74)80241-x. View

Silverman P, Epstein P . Cyclic nucleotide metabolism coupled to cytodifferentiation of Blastocladiella emersonii. Proc Natl Acad Sci U S A. 1975; 72(2):442-6. PMC: 432327. DOI: 10.1073/pnas.72.2.442. View

10.

Schlanderer G, Dellweg H . Cyclid AMP and catabolite repression in yeasts, In Schizosaccharomyces pombe glucose lowers both intracellular adenosine 3':5'-monophosphate levels and the activity of catabolite-sensitive enzymes. Eur J Biochem. 1974; 49(1):305-16. DOI: 10.1111/j.1432-1033.1974.tb03835.x. View

11.

Hereford L, Hartwell L . Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis. J Mol Biol. 1974; 84(3):445-61. DOI: 10.1016/0022-2836(74)90451-3. View

12.

Paznokas J, Sypherd P . Respiratory capacity, cyclic adenosine 3',5'-monophosphate, and morphogenesis of Mucor racemosus. J Bacteriol. 1975; 124(1):134-9. PMC: 235874. DOI: 10.1128/jb.124.1.134-139.1975. View

13.

Terenzi H, Flawia M, Tellez-Inon M, Torres H . Control of Neurospora crassa morphology by cyclic adenosine 3', 5'-monophosphate and dibutyryl cyclic adenosine 3', 5'-monophosphate. J Bacteriol. 1976; 126(1):91-9. PMC: 233263. DOI: 10.1128/jb.126.1.91-99.1976. View

14.

Zonneveld B . The effect of glucose and manganese on adenosine-3',5'-monophosphate levels during growth and differentiation of Aspergillus nidulans. Arch Microbiol. 1976; 108(1):41-4. DOI: 10.1007/BF00425091. View

15.

Scott W . Adenosine 3':5'-cyclic monophosphate deficiency in Neurospora crassa. Proc Natl Acad Sci U S A. 1976; 73(9):2995-9. PMC: 430905. DOI: 10.1073/pnas.73.9.2995. View

16.

Uno I, Ueda T, Greengard P . Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. II. Solubilization, purification, and some properties of an endogenous adenosine 3':5'-monophosphate-dependent protein kinase. J Biol Chem. 1977; 252(14):5164-74. View

17.

Nogi Y, Matsumoto K, Toh-E A, Oshima Y . Interaction of super-repressible and dominant constitutive mutations for the synthesis of galactose pathway enzymes in Saccharomyces cerevisiae. Mol Gen Genet. 1977; 152(3):137-44. DOI: 10.1007/BF00268810. View

18.

Walter U, Uno I, Liu A, Greengard P . Identification, characterization, and quantitative measurement of cyclic AMP receptor proteins in cytosol of various tissues using a photoaffinity ligand. J Biol Chem. 1977; 252(18):6494-500. View

19.

Matsumoto K, Toh-E A, Oshima Y . Genetic control of galactokinase synthesis in Saccharomyces cerevisiae: evidence for constitutive expression of the positive regulatory gene gal4. J Bacteriol. 1978; 134(2):446-57. PMC: 222272. DOI: 10.1128/jb.134.2.446-457.1978. View

20.

Rosenberg G, Pall M . Cyclic AMP and cyclic GMP in germinating conidia of Neurospora crassa. Arch Microbiol. 1978; 118(1):87-90. DOI: 10.1007/BF00406079. View