Comparison of an Enzyme Immunoassay with Electron Microscopic Procedures for Detecting Rotavirus

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1982 May 1

PMID 6284793

Citations 42

Authors

A S RUBENSTEIN

M F Miller

Affiliations

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Abstract

The sensitivity and specificity of an enzyme immunoassay (Rotazyme), an ongrid immunoelectron microscopy procedure, and conventional negative stain electron microscopic techniques were compared. By using partially purified human rotavirus and simian rotavirus (SA-11) of known particle concentration, the enzyme immunoassay was essentially equivalent to the immunoelectron microscopic procedure and significantly more sensitive than conventional electron microscopic techniques. The level of sensitivity was approximately 10(6) particles per ml for simian rotavirus SA-11 and 10(7) particles per ml for human rotavirus. In an evaluation of 455 clinical samples by these techniques, a sensitivity of 98% and specificity of 92% were demonstrated. Samples negative by the immunoelectron microscopic procedure and positive by enzyme immunoassay could be confirmed by a blocking assay.

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References

MALHERBE H, STRICKLAND-CHOLMLEY M . Simian virus SA11 and the related O agent. Arch Gesamte Virusforsch. 1967; 22(1):235-45. DOI: 10.1007/BF01240518. View

RUBENSTEIN A, Gravell M, Darlington R . Protein kinase in enveloped herpes simplex virions. Virology. 1972; 50(1):287-90. DOI: 10.1016/0042-6822(72)90374-1. View

Bishop R, Davidson G, HOLMES I, Ruck B . Virus particles in epithelial cells of duodenal mucosa from children with acute non-bacterial gastroenteritis. Lancet. 1973; 2(7841):1281-3. DOI: 10.1016/s0140-6736(73)92867-5. View

Derrick K . Quantitative assay for plant viruses using serologically specific electron microscopy. Virology. 1973; 56(2):652-3. DOI: 10.1016/0042-6822(73)90068-8. View

Miller M, Allen P, DMOCHOWSKI L . Quantitative studies of oncornaviruses in thin sections. J Gen Virol. 1973; 21:57-68. DOI: 10.1099/0022-1317-21-1-57. View

Bishop R, Davidson G, HOLMES I, Ruck B . Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974; 1(7849):149-51. DOI: 10.1016/s0140-6736(74)92440-4. View

Flewett T, Bryden A, Davies H, Woode G, Bridger J, Derrick J . Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet. 1974; 2(7872):61-3. DOI: 10.1016/s0140-6736(74)91631-6. View

Kapikian A, Kim H, Wyatt R, Rodriguez W, Ross S, Cline W . Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science. 1974; 185(4156):1049-53. DOI: 10.1126/science.185.4156.1049. View

Wyatt R, Kapikian A, Thornhill T, Sereno M, Kim H, Chanock R . In vitro cultivation in human fetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children. J Infect Dis. 1974; 130(5):523-8. DOI: 10.1093/infdis/130.5.523. View

10.

Matsuno S, Inouye S, Kono R . Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977; 5(1):1-4. PMC: 274520. DOI: 10.1128/jcm.5.1.1-4.1977. View

11.

Bryden A, Davies H, Thouless M, Flewitt T . Diagnosis of rotavirus infection by cell culture. J Med Microbiol. 1977; 10(1):121-5. DOI: 10.1099/00222615-10-1-121. View

12.

Thouless M, Bryden A, Flewett T, Woode G, Bridger J, Snodgrass D . Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977; 53(4):287-94. DOI: 10.1007/BF01315627. View

13.

Yolken R, Kim H, Clem T, Wyatt R, Kalica A, Chanock R . Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977; 2(8032):263-7. DOI: 10.1016/s0140-6736(77)90951-5. View

14.

Ellens D, de Leeuw P . Enzyme-linked immunosorbent assay for diagnosis of rotavirus infections in calves. J Clin Microbiol. 1977; 6(5):530-2. PMC: 274810. DOI: 10.1128/jcm.6.5.530-532.1977. View

15.

Vandervelde E, Cohen B, Cossart Y . An enzyme-linked immunosorbent-assay test for hepatitis B surface antigen. J Clin Pathol. 1977; 30(8):714-6. PMC: 476530. DOI: 10.1136/jcp.30.8.714. View

16.

Yolken R, Barbour B, Wyatt R, Kalica A, Kapikian A, Chanock R . Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science. 1978; 201(4352):259-62. DOI: 10.1126/science.208150. View

17.

Cukor G, Berry M, Blacklow N . Simplified radioimmunoassay for detection of human rotavirus in stools. J Infect Dis. 1978; 138(6):906-10. DOI: 10.1093/infdis/138.6.906. View

18.

Madeley C . Viruses in the stools. J Clin Pathol. 1979; 32(1):1-10. PMC: 1145558. DOI: 10.1136/jcp.32.1.1. View

19.

Wyatt R, James W, BOHL E, Theil K, Saif L, Kalica A . Human rotavirus type 2: cultivation in vitro. Science. 1980; 207(4427):189-91. DOI: 10.1126/science.6243190. View

20.

Nicolaieff A, Obert G, Van Regenmortel M . Detection of rotavirus by serological trapping on antibody-coated electron microscope grids. J Clin Microbiol. 1980; 12(1):101-4. PMC: 273529. DOI: 10.1128/jcm.12.1.101-104.1980. View