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Comparison of ELISA, SPACE, and Electron Microscopy for the Routine Diagnosis of Rotavirus Infection

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1982 Jan 1
PMID 6277995
Citations 4
Authors
R A Wall
B J Mellars
P LUTON
S Boulding
Affiliations
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Abstract

Previous studies on the serological diagnosis of rotavirus infection have utilised locally produced antibodies. In this study we have compared two commercially produced assays, an ELISA (Rotazyme, Abbott) and a newly developed assay--solid phase aggregation of coupled erythrocytes (SPACE) (Wellcome Research Laboratories), with electron microscopy (EM). The SPACE test appeared less sensitive than EM. The ELISA was shown to be as sensitive as EM but more versatile. Our experience suggests that the ELISA could be successfully incorporated into the routine of any diagnostic laboratory.

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Antibody against adult diarrhoea rotavirus among healthy adult population in China.

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