Routine Diagnosis of Human Rotaviruses in Stools

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1978 Feb 1

PMID 204665

Citations 16

Authors

G Zissis

J P LAMBERT

D De Kegel

Affiliations

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Abstract

Electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. Complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. Absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts.

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