Virus Inhibition Assay for Measurement of Acyclovir Levels in Human Plasma and Urine

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1981 Dec 1

PMID 6275786

Citations 2

Authors

D F Moore

S C Taylor

Y J Bryson

Affiliations

Soon will be listed here.

Abstract

A simple microplate virus inhibition assay which measures the levels of acyclovir in plasma and urine samples from patients was developed. The assay is based upon the inhibition of the cytopathic effect of herpes simplex virus type 1 on human fibroblast cells. The extent of inhibition of virus cytopathic effect, caused by dilutions of samples from patients, allowed determination of acyclovir concentrations to be made. The assay, which measured biological activity, could detect acyclovir levels of greater than or equal to 1.0 microM. Peak and valley levels measured in plasma samples from two patients were comparable to values obtained by radioimmunoassay. The assay is simple, rapid, and quantitative, and it can be adapted to routine use for a large number of specimens.

Citing Articles

Clinical pharmacokinetics of acyclovir.

Laskin O Clin Pharmacokinet. 1983; 8(3):187-201.

PMID: 6342900 DOI: 10.2165/00003088-198308030-00001.

Acyclovir. A review of its pharmacodynamic properties and therapeutic efficacy.

Richards D, Carmine A, Brogden R, Heel R, Speight T, Avery G Drugs. 1983; 26(5):378-438.

PMID: 6315332 DOI: 10.2165/00003495-198326050-00002.

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