Ubiquitin - Protein Conjugates

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 1981 Nov 13

PMID 6275256

Citations 29

Authors

H Busch

I L Goldknopf

Affiliations

Soon will be listed here.

Abstract

The data available at present indicates there are three distinct functions of ubiquitin, two of which are related to protein conjugation. The first of these has been extensively studied by our laboratory and others interested in nucleosomes and changes in chromatin states. The ubiquitin-histone (Ub-2A, Ub-2B) conjugation reaction now appears to be a very dynamic process. In the deconjugation (lyase) reaction, both the histone 2A and the ubiquitin are left intact and in a form which makes possible ready reconjugation. Accordingly, this may be a mechanism for "moment-to-moment" Control of the genome. The second function in which ubiquitin is conjugated involves proteolytic activity. This activity is correlated with protein turnover. In this process, the ubiquitin-protein conjugate apparently serves as a "signal" for the protease cleavage of the protein. The released ubiquitin is also intact and is probably available for reconjugation. In the third function, ubiquitin was suggested to serve as a "hormone". The studies thus far have been carried out primarily on induction of T- and B-lymphocytes, reduction or delay of Coombs' positivity and reduction of spleen weight. The precise physiological role of this reported function is still unclear, particularly because the ubiquitin used was probably not the physiologically active form.

Citing Articles

APC/C-mediated ubiquitylation of extranucleosomal histone complexes lacking canonical degrons.

Skrajna A, Bodrug T, Martinez-Chacin R, Fisher C, Welsh K, Simmons H Nat Commun. 2025; 16(1):2561.

PMID: 40089476 DOI: 10.1038/s41467-025-57384-7.

Atypical Ubiquitination and Parkinson's Disease.

Buneeva O, Medvedev A Int J Mol Sci. 2022; 23(7).

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Activation and regulation of H2B-Ubiquitin-dependent histone methyltransferases.

Worden E, Wolberger C Curr Opin Struct Biol. 2019; 59:98-106.

PMID: 31229920 PMC: 6888998. DOI: 10.1016/j.sbi.2019.05.009.

E3 ligases determine ubiquitination site and conjugate type by enforcing specificity on E2 enzymes.

David Y, Ternette N, Edelmann M, Ziv T, Gayer B, Sertchook R J Biol Chem. 2011; 286(51):44104-44115.

PMID: 21965653 PMC: 3243545. DOI: 10.1074/jbc.M111.234559.

Detection of ubiquityl-calmodulin conjugates with a novel high-molecular weight ubiquitylprotein-isopeptidase in rabbit tissues.

Sixt S, Jennissen H, Winterhalter M, Laub M Eur J Med Res. 2010; 15(10):428-47.

PMID: 21156402 PMC: 3352187. DOI: 10.1186/2047-783x-15-10-428.

References

Goldknopf I, Wilson G, Ballal N, Busch H . Chromatin conjugate protein A24 is cleaved and ubiquitin is lost during chicken erythropoiesis. J Biol Chem. 1980; 255(22):10555-8. View

Chen R . Isopeptide linkage between N-alpha-monomethylalanine and lysine in ribosomal protein S11 from Escherichia coli. Proc Natl Acad Sci U S A. 1977; 74(11):4905-8. PMC: 432065. DOI: 10.1073/pnas.74.11.4905. View

Schlesinger D, Goldstein G, Scheid M, Bitensky M . Chemical synthesis of a hexadecapeptide segment of ubiquitin that activates adenylate cyclase and induces lymphocytes to differentiate. Experientia. 1978; 34(6):703-4. DOI: 10.1007/BF01947269. View

Boulikas T, WISEMAN J, Garrard W . Points of contact between histone H1 and the histone octamer. Proc Natl Acad Sci U S A. 1980; 77(1):127-31. PMC: 348221. DOI: 10.1073/pnas.77.1.127. View

Goldknopf I, Busch H . Isopeptide linkage between nonhistone and histone 2A polypeptides of chromosomal conjugate-protein A24. Proc Natl Acad Sci U S A. 1977; 74(3):864-8. PMC: 430507. DOI: 10.1073/pnas.74.3.864. View

Kuehl L, Lyness T, DIXON G . Distribution of high mobility group proteins among domains of trout testis chromatin differing in their susceptibility to micrococcal nuclease. J Biol Chem. 1980; 255(3):1090-5. View

Matacic S, Loewy A . The identification of isopeptide crosslinks in insoluble fibrin. Biochem Biophys Res Commun. 1968; 30(4):356-62. DOI: 10.1016/0006-291x(68)90750-x. View

Sugano N, Olson M, Yeoman L, Johnson B, Taylor C, Starbuck W . Amino acid sequence of the COOH-terminal portion of the arginine-lysine-rich histone of calf thymus. J Biol Chem. 1972; 247(11):3589-91. View

Okayama H, HAYAISHI O . ADP-ribosylation of nuclear protein A24. Biochem Biophys Res Commun. 1978; 84(3):755-62. DOI: 10.1016/0006-291x(78)90769-6. View

10.

West M, Bonner W . Histone 2A, a heteromorphous family of eight protein species. Biochemistry. 1980; 19(14):3238-45. DOI: 10.1021/bi00555a022. View

11.

ANDERSEN M, Ballal N, Busch H . Nucleoli of thioacetamide-treated liver as a model for studying regulation of preribosomal RNA synthesis. Biochem Biophys Res Commun. 1977; 78(1):129-35. DOI: 10.1016/0006-291x(77)91230-x. View

12.

Goldstein G, Scheid M, Hammerling U, Schlesinger D, Niall H, Boyse E . Isolation of a polypeptide that has lymphocyte-differentiating properties and is probably represented universally in living cells. Proc Natl Acad Sci U S A. 1975; 72(1):11-5. PMC: 432229. DOI: 10.1073/pnas.72.1.11. View

13.

Wilkinson K, Urban M, Haas A . Ubiquitin is the ATP-dependent proteolysis factor I of rabbit reticulocytes. J Biol Chem. 1980; 255(16):7529-32. View

14.

Gershwin M, Kruse W, Goldstein G . The effect of thymopoietin32-36 and ubiquitin on spontaneous immunopathology of New Zealand mice. J Rheumatol. 1979; 6(6):610-20. View

15.

Bonner W, Stedman J . Histone 1 is proximal to histone 2A and to A24. Proc Natl Acad Sci U S A. 1979; 76(5):2190-4. PMC: 383563. DOI: 10.1073/pnas.76.5.2190. View

16.

West M, Bonner W . Histone 2B can be modified by the attachment of ubiquitin. Nucleic Acids Res. 1980; 8(20):4671-80. PMC: 324378. DOI: 10.1093/nar/8.20.4671. View

17.

Klostermeyer H, Rabbel K, Reimerdes E . [Nepsilon-(beta-Aspartyl)lysine and Nepsilon-(gamma-glutamyl)lysine crosslinkings in bovine colostrum (author's transl)]. Hoppe Seylers Z Physiol Chem. 1976; 357(8):1197-9. View

18.

Brand A, Gilmour D, Goldstein G . Effects of a nonapeptide FTS on lymphocyte differentiations in vitro. Nature. 1977; 269(5629):597-8. DOI: 10.1038/269597a0. View

19.

Seidah N, Crine P, Benjannet S, SCHERRER H, Chretien M . Isolation and partial characterization of a biosynthetic N-terminal methionyl peptide of bovine pars intermedia: relationship to ubiquitin. Biochem Biophys Res Commun. 1978; 80(3):600-8. DOI: 10.1016/0006-291x(78)91611-x. View

20.

Bradbury A, Smyth D, Snell C . Lipotropin: precursor to two biologically active peptides. Biochem Biophys Res Commun. 1976; 69(4):950-6. DOI: 10.1016/0006-291x(76)90465-4. View