Cytological Evidence for Activation of Neuroendocrine Cells in the Parvocellular Preoptic Nucleus of the Goldfish Hypothalamus Following Pharmacological Adrenalectomy

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1981 Jan 1

PMID 6265096

Citations 3

Authors

J N Fryer

Affiliations

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Abstract

The nucleus preopticus (NPO) of the goldfish hypothalamus is composed of parvocellular (NPOpc) and magnocellular (NPOmc) neurosecretory neutrons. The cytology of NPOpc and NPOmc neurons was examined with light and electron microscopy following pharmacological adrenalectomy with the adrenocortical inhibitor, metopirone. After five days of metopirone administration, light microscopy revealed a significant increase in nuclear area of NPOpc, but not of NPOmc, neurons. Ultrastructural examination of NPOpc neurons revealed two cell types, PC1 and PC2 neurons, which could be distinguished by the relative abundance and the size of the neurosecretory granules in the cytoplasm. The ultrastructural appearance of the NPOmc neurons revealed a single cell type containing abundant neurosecretory granules. Following five days of metopirone administration, the ultrastructural appearance of the PC1 neurons indicated a state of enhanced secretory activity. Metopirone had no observable effect on the appearance of the PC2 or NPOmc neurons. These observations demonstrate that PC1 neurons are activated under the conditions of pharmacological adrenalectomy and suggest that the secretory activity of these neurons is inhibited by adrenocorticosteroids.

Citing Articles

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References

Fryer J, Maler L . Hypophysiotropic neurons in the goldfish hypothalamus demonstrated by retrograde transport of horseradish peroxidase. Cell Tissue Res. 1981; 218(1):93-102. DOI: 10.1007/BF00210094. View

CHATEAU M, Marchetti J, Burlet A, BOULANGE M . Evidence of vasopressin in adenohypophysis: research into its role in corticotrope activity. Neuroendocrinology. 1979; 28(1):25-35. DOI: 10.1159/000122841. View

Batten T, Ingleton P, BALL J . Ultrastructural and formaldehyde-fluorescence studies on the hypothalamus of Poecilia latipinna (Teleostei, Cyprinodontiformes). Gen Comp Endocrinol. 1979; 39(1):87-109. DOI: 10.1016/0016-6480(79)90195-3. View

Fryer J, Peter R . Hypothalamic control ACTH secretion in goldfish. II. Hypothalamic lesioning studies. Gen Comp Endocrinol. 1977; 33(2):202-14. DOI: 10.1016/0016-6480(77)90245-3. View

Stillman M, Recht L, Rosario S, Seif S, Robinson A, Zimmerman E . The effects of adrenalectomy and glucocorticoid replacement on vasopressin and vasopressin-neurophysin in the zona externa of the median eminence of the rat. Endocrinology. 1977; 101(1):42-9. DOI: 10.1210/endo-101-1-42. View

Fryer J . Hypothalamic lesions stimulating growth hormone cell activity in the goldfish. Cell Tissue Res. 1981; 214(2):387-95. DOI: 10.1007/BF00249219. View

Silverman A, Gadde C, Zimmerman E . Effects of adrenalectomy on the incorporation of 3H-cytidine in neurophysin and vasopressin-containing neurons of the rat hypothalamus. Neuroendocrinology. 1980; 30(5):285-90. DOI: 10.1159/000123016. View

Fryer J, Peter R . Hypothalamic control of ACTH secretion in goldfish. III. Hypothalamic cortisol implant studies. Gen Comp Endocrinol. 1977; 33(2):215-25. DOI: 10.1016/0016-6480(77)90246-5. View

PALAY S . The fine structure of secretory neurons in the preoptic nucleus of the goldish (Carassius auratus). Anat Rec. 1960; 138:417-43. DOI: 10.1002/ar.1091380404. View

10.

Goossens N, Dierickx K, VANDESANDE F . Immunocytochemical localization of vasotocin and isotocin in the preopticohypophysial neurosecretory system of teleosts. Gen Comp Endocrinol. 1977; 32(4):371-5. DOI: 10.1016/0016-6480(77)90216-7. View

11.

Zimmerman E, Carmel P, Husain M, Ferin M, TANNENBAUM M, FRANTZ A . Vasopressin and neurophysin: high concentrations in monkey hypophyseal portal blood. Science. 1973; 182(4115):925-7. DOI: 10.1126/science.182.4115.925. View

12.

Terlou M, Ekengren B . Nucleus praeopticus and nucleus lateralis tuberis of Salmo salar and Salmo gairdneri: structure and relationship to the hypophysis. Cell Tissue Res. 1979; 197(1):1-21. DOI: 10.1007/BF00233550. View

13.

Fryer J, Peter R . Hypothalamic control of ACTH secretion in goldfish. I. Corticotrophin-releasing factor activity in teleost brain tissue extracts. Gen Comp Endocrinol. 1977; 33(2):196-201. DOI: 10.1016/0016-6480(77)90244-1. View

14.

Reaves Jr T, HAYWARD J . Immunocytochemical identification of enkephalinergic neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus. Cell Tissue Res. 1979; 200(1):147-51. DOI: 10.1007/BF00236894. View

15.

Kaul S, Vollrath L . The goldfish pituitary. I. Cytology. Cell Tissue Res. 1974; 154(2):211-30. DOI: 10.1007/BF00223165. View

16.

Gillies G, Lowry P . Corticotrophin releasing activity in extracts of the stalk median eminence of Brattleboro rats. J Endocrinol. 1980; 84(1):65-73. DOI: 10.1677/joe.0.0840065. View

17.

Peter R, Nagahama Y . A light and electron microscopic study of the structure of the nucleus preopticus and nucleus lateral tuberis of the goldfish, Carassius auratus. Can J Zool. 1976; 54(9):1423-37. DOI: 10.1139/z76-165. View