Uptake of Plasmid Deoxyribonucleic Acid by Haemophilus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Apr 1

PMID 6260748

Citations 11

Authors

R Gromkova

S Goodgal

Affiliations

Soon will be listed here.

Abstract

The uptake of circular and linear plasmid RSF0885 deoxyribonucleic acids, (DNAs) obtained from Haemophilus parainfluenzae 14, in both homologous and heterologous recipients was studied and compared with that of chromosomal DNA. High concentrations of divalent cations stimulated the uptake of either circular or linear plasmid DNA in H. parainfluenzae 14 competent cells but did not affect the uptake of chromosomal DNA. The biological activity of linear plasmid DNA was similar to that of circular DNA, and the transforming efficiencies for ampicillin resistance of both molecular forms were stimulated by divalent ions. Plasmid DNA was taken up efficiently either with or without the addition of divalent ions but was not biologically active in the heterologous Haemophilus influenzae Rd recipient. Our results suggest that in H. parainfluenzae 14 some of the steps for chromosomal and plasmid DNA uptake are different.

Citing Articles

The cell pole: the site of cross talk between the DNA uptake and genetic recombination machinery.

Kidane D, Ayora S, Sweasy J, Graumann P, Alonso J Crit Rev Biochem Mol Biol. 2012; 47(6):531-55.

PMID: 23046409 PMC: 3490228. DOI: 10.3109/10409238.2012.729562.

tfoX (sxy)-dependent transformation of Aggregatibacter (Actinobacillus) actinomycetemcomitans.

Bhattacharjee M, Fine D, Figurski D Gene. 2007; 399(1):53-64.

PMID: 17561357 PMC: 2080652. DOI: 10.1016/j.gene.2007.04.026.

Bacterial gene transfer by natural genetic transformation in the environment.

Lorenz M, Wackernagel W Microbiol Rev. 1994; 58(3):563-602.

PMID: 7968924 PMC: 372978. DOI: 10.1128/mr.58.3.563-602.1994.

Transformation of Haemophilus influenzae by plasmid RSF0885.

NOTANI N, Setlow J, McCarthy D, Clayton N J Bacteriol. 1981; 148(3):812-6.

PMID: 6975775 PMC: 216279. DOI: 10.1128/jb.148.3.812-816.1981.

Uptake of heterologous DNA by Haemophilus influenzae.

GOODGAL S, Mitchell M J Bacteriol. 1984; 157(3):785-8.

PMID: 6365892 PMC: 215327. DOI: 10.1128/jb.157.3.785-788.1984.

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