Sex-related Differences in the Pattern of Coxsackievirus B-3-induced Immune Spleen Cell Cytotoxicity Against Virus-infected Myofibers

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1981 Apr 1

PMID 6260687

Citations 14

Authors

S A Huber

L P Job

J F Woodruff

Affiliations

Soon will be listed here.

Abstract

Spleen cells from adult BALB/c mice injected intraperitoneally with purified coxsackievirus B-3 were tested for cytotoxicity against 51Cr-labeled syngeneic infected and uninfected myofibers. Both male and female immune cells were active against uninfected targets; this reactivity was evident by day 3 of infection and persisted throughout the first week. However, we observed marked sex-related differences in immune cell cytotoxicities against infected myofibers. Males exhibited a strong T-lymphocyte response 4 to 7 days after infection. In contrast, females exhibited a weak response, and only infrequently were the immune spleen cells of females significantly more reactive against infected myofibers than against uninfected myofibers. The demonstration of a stronger effector cell response against infected myocardial cells in male mice correlates with the observation that clinical adult coxsackievirus B myocarditis and pericarditis occur predominantly in males.

Citing Articles

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References

Mackaness G, Lagrange P, Miller T, Ishibashi T . Feedback inhibition of specifically sensitized lymphocytes. J Exp Med. 1974; 139(3):543-59. PMC: 2139549. DOI: 10.1084/jem.139.3.543. View

Sainani G, Krompotic E, SLODKI S . Adult heart disease due to the Coxsackie virus B infection. Medicine (Baltimore). 1968; 47(2):133-47. DOI: 10.1097/00005792-196803000-00003. View

GRODUMS E, DEMPSTER G . Myocarditis in experimental Coxsackie B-3 infection. Can J Microbiol. 1959; 5:605-15. DOI: 10.1139/m59-074. View

Ramshaw I, Bretscher P, Parish C . Regulation of the immune response. I. Suppression of delayed-type hypersensitivity by T cells from mice expressing humoral immunity. Eur J Immunol. 1976; 6(10):674-9. DOI: 10.1002/eji.1830061003. View

Oberg B, Philipson L . Replication of poliovirus RNA studied by gel filtration and electrophoresis. Eur J Biochem. 1969; 11(2):305-15. DOI: 10.1111/j.1432-1033.1969.tb00774.x. View

Wong C, Woodruff J, Woodruff J . Generation of cytotoxic T lymphocytes during coxsackievirus B-3 infection. I. Model and viral specificity1. J Immunol. 1977; 118(4):1159-64. View

Yoon J, Onodera T, Notkins A . Virus-induced diabetes mellitus. XV. Beta cell damage and insulin-dependent hyperglycemia in mice infected with coxsackie virus B4. J Exp Med. 1978; 148(4):1068-80. PMC: 2185018. DOI: 10.1084/jem.148.4.1068. View

Huber S, Job L, Woodruff J . Lysis of infected myofibers by coxsackievirus B-3-immune T lymphocytes. Am J Pathol. 1980; 98(3):681-94. PMC: 1903528. View

Smith W . Coxsackie B myopericarditis in adults. Am Heart J. 1970; 80(1):34-46. DOI: 10.1016/0002-8703(70)90035-9. View

10.

Lerner A, Wilson F . Virus myocardiopathy. Prog Med Virol. 1973; 15:63-91. View

11.

Wilson F, Miranda Q, CHASON J, Lerner A . Residual pathologic changes following murine coxsackie A and B myocarditis. Am J Pathol. 1969; 55(2):253-65. PMC: 2013494. View

12.

Woodruff J, Woodruff J . Involvement of T lymphocytes in the pathogenesis of coxsackie virus B3 heart disease. J Immunol. 1974; 113(6):1726-34. View

13.

Julius M, Simpson E, Herzenberg L . A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973; 3(10):645-9. DOI: 10.1002/eji.1830031011. View

14.

Yoon J, Austin M, Onodera T, Notkins A . Isolation of a virus from the pancreas of a child with diabetic ketoacidosis. N Engl J Med. 1979; 300(21):1173-9. DOI: 10.1056/NEJM197905243002102. View

15.

CROWELL R, SYVERTON J . The mammalian cell-virus relationship. VI. Sustained infection of HeLa cells by Coxsackie B3 virus and effect on superinfection. J Exp Med. 1961; 113:419-35. PMC: 2137354. DOI: 10.1084/jem.113.2.419. View

16.

Wong C, Woodruff J, Woodruff J . Generation of cytotoxic T lymphocytes during coxsackievirus B-3 infection. III. Role of sex. J Immunol. 1977; 119(2):591-7. View

17.

Kim Y, Huh N, Koren H, Amos D . Natural killing (NK) and antibody-dependent cellular cytotoxicity (ADCC) in specific pathogen-free (SPF) miniature swine and germfree piglets. I. Comparison of NK and ADCC. J Immunol. 1980; 125(2):755-62. View

18.

Woodruff J, KILBOURNE E . The influence of quantitated post-weaning undernutrition on coxsackievirus B3 infection of adult mice. I. Viral persistence and increased severity of lesions. J Infect Dis. 1970; 121(2):137-63. DOI: 10.1093/infdis/121.2.137. View

19.

PLAGER H, Beebe R, Miller J . Coxsackie B-5 pericarditis in pregnancy. Arch Intern Med. 1962; 110:735-8. DOI: 10.1001/archinte.1962.03620230181024. View

20.

Berjivich S, Ressel M . Effect of sex on susceptibility of adult mice to coxsackie B1 virus infection. Arch Gesamte Virusforsch. 1967; 22(1):246-51. DOI: 10.1007/BF01240519. View