Mobile Dispersed Genetic Element MDG1 of Drosophila Melanogaster: Structural Organization

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1980 Nov 25

PMID 6258141

Citations 11

Authors

Y V Ilyin

V G Chmeliauskaite

E V Ananiev

N V Lyubomirskaya

V V. Kulguskin

A A Bayev Jr

G P Georgiev

Affiliations

Soon will be listed here.

Abstract

The whole-length mobile dispersed genetic element mdg1 has been cloned from D. melanogaster genome. It contains DNA fragments described earlier as Dm225 and Dm234, Mdg1 is 7.2 kb long and framed with two direct repeats of 300-400 base pairs each. Mdg1 family is represented by about 25 copies in the genome of flies and by 200 copies in the genome of cultured cell line 67J25D. Virtually all the copies in the genome of D. melanogaster have the same restriction map. Oligo(dA)-oligo(dT) regions were found within mdg1.

Citing Articles

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Structural organization of transposable element mdg4 from Drosophila melanogaster and a nucleotide sequence of its long terminal repeats.

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The 5' termini of RNAs encoded by the transposable element copia.

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Mobile dispersed genetic element MDG1 of Drosophila melanogaster: nucleotide sequence of long terminal repeats.

Kulguskin V, Ilyin Y, Georgiev G Nucleic Acids Res. 1981; 9(14):3451-64.

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References

Denhardt D . A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966; 23(5):641-6. DOI: 10.1016/0006-291x(66)90447-5. View

Sharp P, Sugden B, Sambrook J . Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973; 12(16):3055-63. DOI: 10.1021/bi00740a018. View

Thuring R, Sanders J, Borst P . A freeze-squeeze method for recovering long DNA from agarose gels. Anal Biochem. 1975; 66(1):213-20. DOI: 10.1016/0003-2697(75)90739-3. View

Firtel R, Kindle K, Huxley M . Structural organization and processing of the genetic transcript in the cellular slime mold Dictyostelium discoideum. Fed Proc. 1976; 35(1):13-22. View

Georgiev G, Ilyin Y, Ryskov A, Tchurikov N, Yenikolopov G, Gvozdev V . Isolation of eukaryotic DNA fragments containing structural genes and the adjacent sequences. Science. 1977; 195(4276):394-7. DOI: 10.1126/science.401545. View

Flavell R, van den Berg F, Grosveld G . Isolation and characterization of the oligo(dA-dT) clusters and their flanking DNA segments in the rabbit genome. J Mol Biol. 1977; 115(4):715-35. DOI: 10.1016/0022-2836(77)90111-5. View

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Tchurikov N, Ilyin Y, Ananiev E, Georgiev G . The properties of gene Dm 225, a representative of dispersed repetitive genes in Drosophila melanogaster. Nucleic Acids Res. 1978; 5(6):2169-87. PMC: 342151. DOI: 10.1093/nar/5.6.2169. View

Finnegan D, Rubin G, YOUNG M, Hogness D . Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:1053-63. DOI: 10.1101/sqb.1978.042.01.106. View

10.

Ilyin Y, Tchurikov N, Ananiev E, Ryskov A, Yenikolopov G, Limborska S . Studies on the DNA fragments of mammals and Drosophila containing structural genes and adjacent sequences. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:959-69. DOI: 10.1101/sqb.1978.042.01.097. View

11.

Ananiev E, Gvozdev V, Ilyin Yu V, Tchurikov N, Georgiev G . Reiterated genes with varying location in intercalary heterochromatin regions of Drosophila melanogaster polytene chromosomes. Chromosoma. 1978; 70(1):1-17. DOI: 10.1007/BF00292211. View

12.

Potter S, Brorein Jr W, Dunsmuir P, Rubin G . Transposition of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979; 17(2):415-27. DOI: 10.1016/0092-8674(79)90168-5. View

13.

Strobel E, Dunsmuir P, Rubin G . Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in Drosophila. Cell. 1979; 17(2):429-39. DOI: 10.1016/0092-8674(79)90169-7. View

14.

YOUNG M . Middle repetitive DNA: a fluid component of the Drosophila genome. Proc Natl Acad Sci U S A. 1979; 76(12):6274-8. PMC: 411846. DOI: 10.1073/pnas.76.12.6274. View

15.

Tchurikov N, Zelentsova E, Georgiev G . Clusters containing different mobile dispersed genes in the genome of Drosophila melanogaster. Nucleic Acids Res. 1980; 8(6):1243-58. PMC: 323989. DOI: 10.1093/nar/8.6.1243. View

16.

Bayev Jr A, Krayev A, Lyubomirskaya N, Ilyin Y, Skryabin K, Georgiev G . The transposable element Mdg3 in Drosophila melanogaster is flanked with the perfect direct and mismatched inverted repeats. Nucleic Acids Res. 1980; 8(15):3263-73. PMC: 324151. DOI: 10.1093/nar/8.15.3263. View

17.

Ilyin Y, Chmeliauskaite V, Georgiev G . Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes. Nucleic Acids Res. 1980; 8(15):3439-57. PMC: 324162. DOI: 10.1093/nar/8.15.3439. View

18.

RITOSSA F, Spiegelman S . LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965; 53:737-45. PMC: 221060. DOI: 10.1073/pnas.53.4.737. View