Article: Mobile dispersed genetic element MDG1 of Drosophila melanogaster: transcription pattern

Virtually the whole region of the mobile dispersed genetic element mdg1 including the direct repeats that frame mdg1 /1/ is transcribed. The end of the transcriptional unit is located within the direct repeat. Although the transcription of mdg1 is symmetric, one direction is transcribed preferentially and is a full-length transcript represented by approximately 29S poly(A)+RNA. Besides it, approximately 15S poly(A)+RNA is present in the cytoplasm of culture cells which corresponds to the 3'-part of 29S RNA and possibly to a small region near the 5'-end of the latter. The major fraction of mdg1 transcripts is present in the fraction of free cytoplasmic RNP particles while only 10-20% are recovered in polysomes. In this respect, mdg1 is similar to mdg3 and quite different from two single-copy genes transcripts of which are almost completely recovered in polysomes. The relationship between mobile dispersed genetic elements and endogeneous viral genomes is discussed.

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References

1.

McKenzie S, Henikoff S, Meselson M . Localization of RNA from heat-induced polysomes at puff sites in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1975; 72(3):1117-21. PMC: 432477. DOI: 10.1073/pnas.72.3.1117. View

2.

Mellon P, Duesberg P . Subgenomic, cellular Rous sarcoma virus RNAs contain oligonucleotides from the 3' half and the 5' terminus of virion RNA. Nature. 1977; 270(5638):631-4. DOI: 10.1038/270631a0. View

3.

Rothenberg E, Donoghue D, Baltimore D . Analysis of a 5' leader sequence on murine leukemia virus 21S RNA: heteroduplex mapping with long reverse transcriptase products. Cell. 1978; 13(3):435-51. DOI: 10.1016/0092-8674(78)90318-5. View

4.

Tchurikov N, Ilyin Y, Ananiev E, Georgiev G . The properties of gene Dm 225, a representative of dispersed repetitive genes in Drosophila melanogaster. Nucleic Acids Res. 1978; 5(6):2169-87. PMC: 342151. DOI: 10.1093/nar/5.6.2169. View

5.

Finnegan D, Rubin G, YOUNG M, Hogness D . Repeated gene families in Drosophila melanogaster. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 2:1053-63. DOI: 10.1101/sqb.1978.042.01.106. View

6.

Canaani E, Aaronson S . Restriction enzyme analysis of mouse cellular type C viral DNA: emergence of new viral sequences in spontaneous AKR/J lymphomas. Proc Natl Acad Sci U S A. 1979; 76(4):1677-81. PMC: 383453. DOI: 10.1073/pnas.76.4.1677. View

7.

Gilboa E, Mitra S, Goff S, Baltimore D . A detailed model of reverse transcription and tests of crucial aspects. Cell. 1979; 18(1):93-100. DOI: 10.1016/0092-8674(79)90357-x. View

8.

Gehring W, Paro R . Isolation of a hybrid plasmid with homologous sequences to a transposing element of Drosophila melanogaster. Cell. 1980; 19(4):897-904. DOI: 10.1016/0092-8674(80)90081-1. View

9.

Tchurikov N, Zelentsova E, Georgiev G . Clusters containing different mobile dispersed genes in the genome of Drosophila melanogaster. Nucleic Acids Res. 1980; 8(6):1243-58. PMC: 323989. DOI: 10.1093/nar/8.6.1243. View

10.

Ilyin Y, Chmeliauskaite V, Georgiev G . Double-stranded sequences in RNA of Drosophila melanogaster: relation to mobile dispersed genes. Nucleic Acids Res. 1980; 8(15):3439-57. PMC: 324162. DOI: 10.1093/nar/8.15.3439. View

11.

Ilyin Y, Chmeliauskaite V, Ananiev E, Lyubomirskaya N, Kulguskin V, Bayev Jr A . Mobile dispersed genetic element MDG1 of Drosophila melanogaster: structural organization. Nucleic Acids Res. 1980; 8(22):5333-46. PMC: 324305. DOI: 10.1093/nar/8.22.5333. View

Mobile Dispersed Genetic Element MDG1 of Drosophila Melanogaster: Transcription Pattern