Identification of the DnaA and DnaN Gene Products of Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 6258023

Citations 16

Authors

S Yuasa

Y Sakakibara

Affiliations

Soon will be listed here.

Abstract

A specialized transducing lambda phage carrying the dnaN genes of Escherichia coli specifies two proteins of about 41 and 48 kilodaltons (kd). The temperature-sensitive mutations, dnaN59 and dnaA167, were found to result in altered isoelectric points of the 41 and 48 kd proteins, respectively. Thus the dnaN gene product was identified as a weakly acidic 41 and 48 kd protein. The synthesis of the dnaN gene product is greatly reduced by insertion of a transposon Tn3 in the dnaA gene and by deletion in the gene at the distal end to the dnaN gene. Temperature-sensitive dnaA mutations, on the dnaN gene product. These results indicate that the synthesis of the dnaN gene product is dependent on the structural integrity of the dnaA gene.

Citing Articles

The origin of replication, oriC, and the dnaA protein are dispensable in stable DNA replication (sdrA) mutants of Escherichia coli K-12.

Kogoma T, von Meyenburg K EMBO J. 1983; 2(3):463-8.

PMID: 11894964 PMC: 555155. DOI: 10.1002/j.1460-2075.1983.tb01445.x.

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

Effect of the dnaN mutation on the growth of small DNA phages.

TAKETO A Mol Gen Genet. 1981; 183(1):130-3.

PMID: 6460152 DOI: 10.1007/BF00270150.

Functional cooperation of the dnaE and dnaN gene products in Escherichia coli.

Kuwabara N, Uchida H Proc Natl Acad Sci U S A. 1981; 78(9):5764-7.

PMID: 6458043 PMC: 348855. DOI: 10.1073/pnas.78.9.5764.

The dnaN gene codes for the beta subunit of DNA polymerase III holoenzyme of escherichia coli.

Burgers P, Kornberg A, Sakakibara Y Proc Natl Acad Sci U S A. 1981; 78(9):5391-5.

PMID: 6458041 PMC: 348751. DOI: 10.1073/pnas.78.9.5391.

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