Partial Amino-terminal Sequences of the Polyoma Nonhistone Proteins VP1, VP2, and VP3 Synthesized in Vitro

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1980 Feb 1

PMID 6251238

Citations 5

Authors

R M Hewick

A Mellor

A E Smith

M D Waterfield

Affiliations

Soon will be listed here.

Abstract

The three polyoma virus capsid proteins VP1, VP2, and VP3 were synthesized in vitro in the presence of several radiolabeled amino acids and, after purification on sodium dodecyl sulfate-polyacrylamide gels, were subjected to sequential Edman degradation. The partial amino-terminal amino acid sequences obtained were compared with the sequence of amino acids predicted from the polyoma virus DNA sequencing (Arrand et al., J. Virol. 33:606--618, 1980). Together, these results showed that the 5' ends of the VP1, VP2, and VP3 coding sequences are located 1,217, 289, and 634 nucleotides, respectively, from the junction of HpaII restriction fragments 3 and 5.

Citing Articles

Acetylation of histone-like proteins of adenovirus type 5.

Fedor M, Daniell E J Virol. 1980; 35(3):637-43.

PMID: 7420537 PMC: 288857. DOI: 10.1128/JVI.35.3.637-643.1980.

Localization of three major cappe 5' ends of polyoma virus late mRNA's within a single tetranucleotide sequence in the viral genome.

Flavell A, Cowie A, Arrand J, Kamen R J Virol. 1980; 33(2):902-8.

PMID: 6251254 PMC: 288615. DOI: 10.1128/JVI.33.2.902-908.1980.

Topography of the three late mRNA's of polyoma virus which encode the virion proteins.

Kamen R, Favaloro J, Parker J J Virol. 1980; 33(2):637-51.

PMID: 6251239 PMC: 288588. DOI: 10.1128/JVI.33.2.637-651.1980.

Polyoma virus DNA: complete nucleotide sequence of the gene which codes for polyoma virus capsid protein VP1 and overlaps the VP2/VP3 genes.

Soeda E, Arrand J, Griffin B J Virol. 1980; 33(2):619-30.

PMID: 6251237 PMC: 288586. DOI: 10.1128/JVI.33.2.619-630.1980.

Polyoma virus DNA: Sequence from the late region that specifies the leader sequence for late mRNA and codes for VP2, VP3, and the N-terminus of VP1.

Arrand J, Soeda E, Walsh J, Smolar N, Griffin B J Virol. 1980; 33(2):606-18.

PMID: 6251236 PMC: 288585. DOI: 10.1128/JVI.33.2.606-618.1980.

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