Cell-free Synthesis of Polyoma Virus Capsid Proteins VP1 and VP2

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1977 Jan 1

PMID 189065

Citations 11

Authors

T Wheeler

S T Bayley

R Harvey

L V CRAWFORD

A E Smith

Affiliations

Soon will be listed here.

Abstract

Polyadenylated RNA isolated from the cytoplasm of mouse 3T6 cells 28 h after infection with polyoma virus has been isolated and translated in vitro. Polyoma capsid proteins VP1 and VP2 have been identified in the cell-free product by polyacrylamide gel electrophoresis, specific immunoprecipitation, and tryptic peptide fingerprinting. Polyoma mRNA species have been isolated by preparative hybridization to purified viral DNA immobilized on cellulose nitrate filters and shown to code for both VP1 and VP2. These experiments establish conditions for the isolation of late polyoma mRNA and the cell-free synthesis of polyoma capsid proteins and indicate that the active mRNA species are at least partially virus coded.

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Partial amino-terminal sequences of the polyoma nonhistone proteins VP1, VP2, and VP3 synthesized in vitro.

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