Structure of the Escherichia Coli K2 Capsular Antigen, a Teichoic Acid-like Polymer

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Sep 1

PMID 6251025

Citations 8

Authors

K Jann

B JANN

M A Schmidt

W F Vann

Affiliations

Soon will be listed here.

Abstract

The primary structure of the K2 antigen of Escherichia coli was elucidated by composition, alkaline fragmentation, dephosphorylation with hydrofluoric acid, periodate oxidation, and methylation analysis. The polymer contains galactose in the pyranosidic and furanosidic ring form. It consists of phosphogaolactopyranosyl glycerol and phosphagalactofuranosyl glycerol units in a molar ratio of 2:1. The sequence of these units is not known. The structure of the K2 antigen is reminiscent of that of certain teichoic acids of gram-positive bacteria. Using microprecipitation, it was shown that in the polymer galactoside is immunodominant.

Citing Articles

Bacterial capsular polysaccharides with antibiofilm activity share common biophysical and electrokinetic properties.

Bernal-Bayard J, Thiebaud J, Brossaud M, Beaussart A, Caillet C, Waldvogel Y Nat Commun. 2023; 14(1):2553.

PMID: 37137893 PMC: 10156666. DOI: 10.1038/s41467-023-37925-8.

Two host clades, two bacterial arsenals: evolution through gene losses in facultative endosymbionts.

Rollat-Farnier P, Santos-Garcia D, Rao Q, Sagot M, Silva F, Henri H Genome Biol Evol. 2015; 7(3):839-55.

PMID: 25714744 PMC: 5322557. DOI: 10.1093/gbe/evv030.

Anti-biofilm activity of an exopolysaccharide from a sponge-associated strain of Bacillus licheniformis.

Abu Sayem S, Manzo E, Ciavatta L, Tramice A, Cordone A, Zanfardino A Microb Cell Fact. 2011; 10:74.

PMID: 21951859 PMC: 3196911. DOI: 10.1186/1475-2859-10-74.

Antibiofilm activity of an exopolysaccharide from marine bacterium Vibrio sp. QY101.

Jiang P, Li J, Han F, Duan G, Lu X, Gu Y PLoS One. 2011; 6(4):e18514.

PMID: 21490923 PMC: 3072402. DOI: 10.1371/journal.pone.0018514.

Role of the K2 capsule in Escherichia coli urinary tract infection and serum resistance.

Buckles E, Wang X, Lane M, Lockatell C, Johnson D, Rasko D J Infect Dis. 2009; 199(11):1689-97.

PMID: 19432551 PMC: 3872369. DOI: 10.1086/598524.

References

Burger M, Glaser L . The synthesis of teichoic acids. V. Polyglucosylglycerol phosphate and polygalactosylglycerol phosphate. J Biol Chem. 1966; 241(2):494-506. View

Forrester I, WICKEN A . A novel teichoic acid-like polymer from the cell walls of Bacillus coagulans NRS T2007. Biochem Biophys Res Commun. 1966; 25(1):23-7. DOI: 10.1016/0006-291x(66)90634-6. View

Hungerer D, Jann K, JANN B, ORSKOV F, ORSKOV I . Immunochemistry of K antigens of Escherichia coli. 4. The K antigen of E. coli O 9:K30:H12. Eur J Biochem. 1967; 2(1):115-26. DOI: 10.1111/j.1432-1033.1967.tb00115.x. View

Schlecht S, Westphal O . [Growth and lipopolysaccharide (O-antigen) levels of salmonella in cultures of agar media]. Zentralbl Bakteriol Orig. 1966; 200(2):241-59. View

Archibald A, BADDILEY J, BLUMSOM N . The teichoic acids. Adv Enzymol Relat Areas Mol Biol. 1968; 30:223-53. DOI: 10.1002/9780470122754.ch5. View

Kochetkov N, Chizhov O . Mass spectrometry of carbohydrate derivatives. Adv Carbohydr Chem Biochem. 1966; 21:39-93. DOI: 10.1016/s0096-5332(08)60315-x. View

JANN B, Jann K, Schmidt G, ORSKOV I, ORSKOV F . Immunochemical studies of polysaccharide surface antigens of Escherichia coli 0100:K?(B):H2. Eur J Biochem. 1970; 15(1):29-39. DOI: 10.1111/j.1432-1033.1970.tb00972.x. View

ORSKOV F, ORSKOV I, JANN B, Jann K . Immunoelectrophoretic patterns of extracts from all Escherichia coli O and K antigen test strains: correlation with pathogenicity. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971; 79(2):142-52. DOI: 10.1111/j.1699-0463.1971.tb02141.x. View

Reske K, Jann K . The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1972; 31(2):320-8. DOI: 10.1111/j.1432-1033.1972.tb02536.x. View

10.

Gotschlich E, Rey M, Etienne J, SANBORN W, Triau R, CVJETANOVIC B . The immunological responses observed in field studies in Africa with group A meningococcal vaccines. Prog Immunobiol Stand. 1972; 5:485-91. View

11.

Kaijser B, Jodal U, Hanson L . Studies on antibody response and tolerance to E, coli K antigens in immunized rabbits and in children with urinary tract infection. Int Arch Allergy Appl Immunol. 1973; 44(2):260-73. DOI: 10.1159/000230935. View

12.

Kaijser B, Olling S . Experimental hematogenous pyelonephritis due to Escherichia coli in rabbits: the antibody response and its protective capacity. J Infect Dis. 1973; 128(1):41-9. DOI: 10.1093/infdis/128.1.41. View

13.

KNOX K, WICKEN A . Immunological properties of teichoic acids. Bacteriol Rev. 1973; 37(2):215-57. PMC: 413812. DOI: 10.1128/br.37.2.215-257.1973. View

14.

Curvall M, Lindberg B, Lonngren J, NIMMICH W . Structural studies on the Klebsiella O group 3 lipopolysaccharide. Acta Chem Scand. 1973; 27(7):2645-9. DOI: 10.3891/acta.chem.scand.27-2645. View

15.

Jodal U, Ahlstedt S, Carlsson B, Hanson L, Lindberg U, Sohl A . Local antibodies in childhood urinary tract infection: a preliminary study. Int Arch Allergy Appl Immunol. 1974; 47(4):537-46. DOI: 10.1159/000231248. View

16.

Prehm P, STIRM S, JANN B, Jann K . Cell-wall lipopolysaccharide from Escherichia coli B. Eur J Biochem. 1975; 56(1):41-55. DOI: 10.1111/j.1432-1033.1975.tb02205.x. View

17.

Bracha R, Glaser L . In vitro system for the synthesis of teichoic acid linked to peptidoglycan. J Bacteriol. 1976; 125(3):872-9. PMC: 236161. DOI: 10.1128/jb.125.3.872-879.1976. View

18.

Prehm P, JANN B, Jann K . The O9 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1976; 67(1):53-6. DOI: 10.1111/j.1432-1033.1976.tb10631.x. View

19.

Vann W, Liu T, Robbins J . Bacillus pumilus polysaccharide cross-reactive with meningococcal group A polysaccharide. Infect Immun. 1976; 13(6):1654-62. PMC: 420816. DOI: 10.1128/iai.13.6.1654-1662.1976. View

20.

Kaijser B, Hanson L, Jodal U, Robbins J . Frequency of E. coli K antigens in urinary-tract infections in children. Lancet. 1977; 1(8013):663-6. DOI: 10.1016/s0140-6736(77)92111-0. View