Bacillus Pumilus Polysaccharide Cross-reactive with Meningococcal Group A Polysaccharide

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1976 Jun 1

PMID 184043

Citations 26

Authors

W F Vann

T Y Liu

J B Robbins

Affiliations

Soon will be listed here.

Abstract

A polysaccharide, antigenically and structurally related to meningococcal group A polysaccharide, was isolated from Bacillus pumilus Sh-17. This enteric bacterium has been implicated as a source of natural meningococcal group A immunity (Myerowitz et al., 1973). The B. pumilus polysaccharide was composed of a homopolymer of (1-6)-N-acetyl-manosamine-1-phosphate, glycerol phosphate teichoic acid-containing N-acetylglucosamine and alkali-labile alanine esters, and a mucopeptide. The cross-reaction was due to the poly-(1-6)-N-acetyl-mannosamine-1-phosphate in the B. pumilus and the meningococcal group A polysaccharides, based on the following evidence. Both polysaccharides contained N-acetyl-mannosamine phosphate. Periodate oxidized the mannosamine phosphate residues of the polysaccharide and destroyed their precipitating activity with meningococcal group A antiserum. Mild acid treatment released phosphomonoesters and destroyed the meningococcal group A precipitating activity of both polysaccharides. N-acetyl-mannosamine-6-phosphate inhibited the precipitation reaction between strain Sh-17 and meningococcal group A antisera. Only mannosamine phosphate was detected in trichloroacetic acid extracts of Sh-17 polysaccharide and meningococcal group A antigen-antibody precipitates.

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References

Heyndrickx A . Paper chromatography of choline and the vitamins B1, B2, niacin and niacinamide; preparation of radioactive choline acetate and study of its hydrolysis. J Am Pharm Assoc Am Pharm Assoc. 1953; 42(11):680-1. DOI: 10.1002/jps.3030421111. View

Torii M, KABAT E, BEZER A . SEPARATION OF TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS INTO TWO IMMUNOLOGICALLY DISTINCT SPECIFIC POLYSACCHARIDES WITH ALPHA- AND BETA-N-ACETYLGLUCOSAMINYL LINKAGES RESPECTIVELY. ANTIGENICITY OF THEICHOIC ACIDS IN MAN. J Exp Med. 1964; 120:13-29. PMC: 2137721. DOI: 10.1084/jem.120.1.13. View

Sharon N, JEANLOZ R . The diaminohexose component of a polysaccharide isolated from Bacillus subtilis. J Biol Chem. 1960; 235:1-5. View

Park J, Johnson M . A submicrodetermination of glucose. J Biol Chem. 1949; 181(1):149-51. View

Robbins J, Myerowitz L, Whisnant J, ARGAMAN M, Schneerson R, Handzel Z . Enteric bacteria cross-reactive with Neisseria meningitidis groups A and C and Diplococcus pneumoniae types I and 3. Infect Immun. 1972; 6(5):651-6. PMC: 422589. DOI: 10.1128/iai.6.5.651-656.1972. View

Myerowitz R, Gordon R, Robbins J . Polysaccharides of the genus Bacillus cross-reactive with the capsular polysaccharides of Diplococcus pneumoniae type 3, Haemophilus influenzae type b, and Neisseria meningitidis group A. Infect Immun. 1973; 8(6):896-900. PMC: 422947. DOI: 10.1128/iai.8.6.896-900.1973. View

Feldman H . Meningococcal infections. Adv Intern Med. 1972; 18:117-40. View

ARGAMAN M, Liu T, Robbins J . Polyribitol-phosphate: an antigen of four gram-positive bacteria cross-reactive with the capsular polysaccharide of haemophilus influenzae type B. J Immunol. 1974; 112(2):649-55. View

Liu T, Gotschlich E . Muramic acid phosphate as a component of the mucopeptide of Gram-positive bacteria. J Biol Chem. 1967; 242(3):471-6. View

10.

Distler J, Kaufman B, Roseman S . Enzymic synthesis of a diamino sugar nucleotide by extracts of type XIV Diplococcus pneumoniae. Arch Biochem Biophys. 1966; 116(1):466-78. DOI: 10.1016/0003-9861(66)90054-3. View

11.

Tomasz A . Choline in the cell wall of a bacterium: novel type of polymer-linked choline in Pneumococcus. Science. 1967; 157(3789):694-7. DOI: 10.1126/science.157.3789.694. View

12.

Brundish D, BADDILEY J . Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. Biochem J. 1968; 110(3):573-82. PMC: 1187388. DOI: 10.1042/bj1100573. View

13.

Archibald A, BADDILEY J . The teichoic acids. Adv Carbohydr Chem Biochem. 1966; 21:323-75. DOI: 10.1016/s0096-5332(08)60320-3. View

14.

Gotschlich E, Liu T, Artenstein M . Human immunity to the meningococcus. 3. Preparation and immunochemical properties of the group A, group B, and group C meningococcal polysaccharides. J Exp Med. 1969; 129(6):1349-65. PMC: 2138651. DOI: 10.1084/jem.129.6.1349. View

15.

Liu T, Gotschlich E, Jonssen E, Wysocki J . Studies on the meningococcal polysaccharides. I. Composition and chemical properties of the group A polysaccharide. J Biol Chem. 1971; 246(9):2849-58. View

16.

Liu T, Chang Y . Hydrolysis of proteins with p-toluenesulfonic acid. Determination of tryptophan. J Biol Chem. 1971; 246(9):2842-8. View