Identification and Molecular Cloning of Moloney Mouse Sarcoma Virus-specific Sequences from Uninfected Mouse Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1980 May 1

PMID 6248858

Citations 29

Authors

M Jones

R A Bosselman

F A van der Hoorn

A Berns

H Fan

I M Verma

Affiliations

Soon will be listed here.

Abstract

When uninfected mouse cell DNA is cleaved with restriction endonuclease EcoRI, a DNA fragment of 14.0 kilobases can be identified by hybridization to cloned DNA containing sarcoma specific sequences of Moloney mouse sarcoma virus (M-MSVsrc). The cellular DNA fragment contains the entire M-MSVsrc specific sequences. The 14.0-kilobase EcoRI DNA fragment was cloned in bacteriophage lambda. The sequence organization of a recombinant clone, lambda . MTX-1, was analyzed by restriction endonuclease mapping, nuclease S1 mapping, and electron microscopy. The results indicate that lambda . MTX-1 contains an uninterrupted stretch of 1.0 kilobase similar to that found in the M-MSV genome.

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