Pausing and Termination of Human RNA Polymerase II Transcription at a Procaryotic Terminator

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1983 Oct 1

PMID 6227805

Citations 6

Authors

G W Hatfield

J A Sharp

M Rosenberg

Affiliations

Soon will be listed here.

Abstract

Kinetic analyses of runoff transcription in a cell-free eucaryotic transcription system revealed that the bacteriophage lambda 4S RNA terminator caused human RNA polymerase II to pause on the template and partially terminate transcription of transcripts initiated by the adenovirus 2 major late promoter. Analogous to the procaryotic RNA polymerase, the eucaryotic enzyme terminated just beyond the guanine-plus-cytosine-rich region of dyad symmetry in the terminator sequence. These results suggest that the eucaryotic RNA polymerase II may respond to transcription termination sequences similar to those used by the procaryotic enzyme. However, similar templates containing lambda tint or lambda tR1 terminators did not elicit pausing or termination, suggesting that other features, such as sequence specificity, may also be involved.

Citing Articles

Sequences responsible for transcription termination on a gene segment in Saccharomyces cerevisiae.

Henikoff S, COHEN E Mol Cell Biol. 1984; 4(8):1515-20.

PMID: 6436686 PMC: 368942. DOI: 10.1128/mcb.4.8.1515-1520.1984.

Attenuation in SV40 as a mechanism of transcription-termination by RNA polymerase B.

Hay N, Aloni Y Nucleic Acids Res. 1984; 12(3):1401-14.

PMID: 6322106 PMC: 318585. DOI: 10.1093/nar/12.3.1401.

Pausing and premature termination of human RNA polymerase II during transcription of adenovirus in vivo and in vitro.

Maderious A, Chen-Kiang S Proc Natl Acad Sci U S A. 1984; 81(19):5931-5.

PMID: 6091120 PMC: 391832. DOI: 10.1073/pnas.81.19.5931.

Specific termination of in vitro transcription by calf thymus RNA polymerase III.

Watson J, Chandler D, Gralla J Nucleic Acids Res. 1984; 12(13):5369-84.

PMID: 6087284 PMC: 318925. DOI: 10.1093/nar/12.13.5369.

Five mouse tubulin isotypes and their regulated expression during development.

Lewis S, Lee M, Cowan N J Cell Biol. 1985; 101(3):852-61.

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