Induction of Suppressor Macrophages in Mice by Fusarenon-X

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1982 Dec 1

PMID 6216202

Citations 2

Authors

E Masuda

T Takemoto

T Tatsuno

T Obara

Affiliations

Soon will be listed here.

Abstract

Intraperitoneal injection of Fusarenon-X into BALB/c mice, a mycotoxin produced by Fusarium nivale Fn 2B, depressed polyclonal antibody formation of splenic lymphocytes in response to pokeweed mitogen (PWM). This inhibitory activity was found to reside in the surface immunoglobulin-negative spleen cell fraction of Fusarenon-X-treated mice, sIg-(FX), which comprised mainly T lymphocytes and smaller number of non-lymphocytic cellular elements. However, reconstitution experiments for in vitro antibody formation provided evidence that T lymphocytes from Fusarenon-X-treated mice, T(FX), which were separated from non-lymphocytic cells by use of carbonyl iron/magnet, were as effective as T lymphocytes from normal mice, T(N), in supporting antibody formation. Furthermore, addition of non-lymphocytic cells, NL(FX), or adherent cells, AD(FX), prepared from spleen cells of Fusarenon-X-treated mice to normal spleen cells strongly inhibited in vitro antibody formation against PWM or a bacterial lipopolysaccharide (LPS). These results strongly indicated that Fusarenon-X induced non-lymphocytic suppressor cells in the spleen of the treated mice which had features in common with activated macrophages.

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References

Tatsuno T . Toxicologic research on substances fro Fusarium nivale. Cancer Res. 1968; 28(11):2393-6. View

Fujimoto Y, Morita Y, Tatsuno T . [Toxicologic study on the toxic substances of Fusarium nivale: chemical study of principal toxins, nivalenol, fusarenon-X and nivalenol-4,15-di-O-acetate]. Chem Pharm Bull (Tokyo). 1972; 20(6):1194-203. DOI: 10.1248/cpb.20.1194. View

Scott M . Biological effects of the adjuvant Corynebacterium parvum. II. Evidence for macrophage-T-cell interaction. Cell Immunol. 1972; 5(3):469-79. DOI: 10.1016/0008-8749(72)90073-1. View

Kirchner H, Chused T, Herberman R, HOLDEN H, LAVRIN D . Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Moloney sarcoma virus. J Exp Med. 1974; 139(6):1473-87. PMC: 2139691. DOI: 10.1084/jem.139.6.1473. View

Kirchner H, Herberman R, Glaser M, LAVRIN D . Suppression of in vitro lymphocyte stimulation in mice bearing primary Moloney sarcoma virus-induced tumors. Cell Immunol. 1974; 13(1):32-40. DOI: 10.1016/0008-8749(74)90224-x. View

Glaser M, Kirchner H, Herberman R . Inhibition of in vitro lymphoproliferative responses to tumor-associated antigens by suppressor cells from rats bearing progressively growing Gross leukemia virus-induced tumors. Int J Cancer. 1975; 16(3):384-93. DOI: 10.1002/ijc.2910160305. View

Kirchner H, HOLDEN H, Herberman . Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975; 115(5):1212-6. View

Glaser M, Kirchner H, HOLDEN H, Herberman R . Inhibition of cell-mediated cytotoxicity against tumor-associated antigens by suppressor cells from tumor-bearing mice. J Natl Cancer Inst. 1976; 56(4):865-7. DOI: 10.1093/jnci/56.4.865. View

Veit B, FELDMAN J . Altered lymphocyte functions in rats bearing syngeneic Moloney sarcoma tumors. I. Mitogen responses, mixed lymphocyte reactions (MLR) and mixed lymphocyte-tumor reactions (MLTR). J Immunol. 1976; 117(2):646-54. View

10.

Veit B, FELDMAN J . Altered lymphocyte functions in rats bearing syngeneic Moloney sarcoma tumors. II. Suppressor cells. J Immunol. 1976; 117(2):655-60. View

11.

Oehler J, Campbell Jr D, Herberman R . In vitro inhibition of lymphoproliferative responses to tumor associated antigens and of lymphoma cell proliferation by rat splenic macrophages. Cell Immunol. 1977; 28(2):355-70. DOI: 10.1016/0008-8749(77)90118-6. View

12.

Wysocki L, Sato V . "Panning" for lymphocytes: a method for cell selection. Proc Natl Acad Sci U S A. 1978; 75(6):2844-8. PMC: 392661. DOI: 10.1073/pnas.75.6.2844. View

13.

Grimm W, Seitz M, Kirchner H, Gemsa D . Prostaglandin synthesis in spleen cell cultures of mice injected with Corynebacterium parvum. Cell Immunol. 1978; 40(2):419-26. DOI: 10.1016/0008-8749(78)90349-0. View

14.

Nathan C, Silverstein S, Brukner L, COHN Z . Extracellular cytolysis by activated macrophages and granulocytes. II. Hydrogen peroxide as a mediator of cytotoxicity. J Exp Med. 1979; 149(1):100-13. PMC: 2184732. DOI: 10.1084/jem.149.1.100. View

15.

Nathan C, Brukner L, Silverstein S, COHN Z . Extracellular cytolysis by activated macrophages and granulocytes. I. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. J Exp Med. 1979; 149(1):84-99. PMC: 2184749. DOI: 10.1084/jem.149.1.84. View

16.

Rosenstein Y, Lafarge-Frayssinet C, Lespinats G, LOISILLIER F, LAFONT P, Frayssinet C . Immunosuppressive activity of Fusarium toxins. Effects on antibody synthesis and skin grafts of crude extracts, T2-toxin and diacetoxyscirpenol. Immunology. 1979; 36(1):111-7. PMC: 1457389. View

17.

Metzger Z, Hoffeld J, Oppenheim J . Macrophage-mediated suppression. I. Evidence for participation of both hdyrogen peroxide and prostaglandins in suppression of murine lymphocyte proliferation. J Immunol. 1980; 124(2):983-8. View

18.

Bixler Jr G, Booss J . Adherent spleen cells from mice acutely infected with cytomegalovirus suppress the primary antibody response in vitro. J Immunol. 1981; 127(4):1294-9. View

19.

Masuda E, Takemoto T, Tatsuno T, Obara T . Immunosuppressive effect of a trichothecene mycotoxin, Fusarenon-X in mice. Immunology. 1982; 45(4):743-9. PMC: 1555432. View