Escherichia Coli O18ac Antigen: Structure of the O-specific Polysaccharide Moiety

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1984 Jul 1

PMID 6203837

Citations 7

Authors

D S Gupta

B JANN

K Jann

Affiliations

Soon will be listed here.

Abstract

The O-specific polysaccharide moiety (O18ac polysaccharide) of the O18ac antigen (lipopolysaccharide) from Escherichia coli 2980 (O18ac:K5:Fim+:H-) was isolated in pure form by degradation of the lipopolysaccharide and chromatography on Sephadex G-50. The primary structure of the O18ac polysaccharide was elucidated by composition, fragmentation procedures, methylation analysis, and nuclear magnetic resonance spectroscopy. The polysaccharide consists of repeating units of the pentasaccharide: (formula; see text) which are joined in the polymer by alpha-1,2 linkages.

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References

ORSKOV I, ORSKOV F, JANN B, Jann K . Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977; 41(3):667-710. PMC: 414020. DOI: 10.1128/br.41.3.667-710.1977. View

JANN B, Prehm P, Jann K . Citrobacter O-antigens: structure of the O-antigenic polysaccharide from Citrobacter sp. 396. J Bacteriol. 1978; 134(2):462-9. PMC: 222274. DOI: 10.1128/jb.134.2.462-469.1978. View

Lindberg B, Lonngren J . Methylation analysis of complex carbohydrates: general procedure and application for sequence analysis. Methods Enzymol. 1978; 50:3-33. DOI: 10.1016/0076-6879(78)50003-7. View

van Halbeek H, Dorland L, Vliegenthart J, Schmid K, Montreuil J, Fournet B . The application of 500-MHz H-NMR spectroscopy for the structure elucidation of N-acetyllactosamine type asparagine-bound carbohydrate chains of glycoproteins. FEBS Lett. 1980; 114(1):11-6. DOI: 10.1016/0014-5793(80)80850-7. View

Wevers P, Picken R, Schmidt G, JANN B, Jann K, Golecki J . Characterization of pili associated with Escherichia coli O18ac. Infect Immun. 1980; 29(2):685-91. PMC: 551181. DOI: 10.1128/iai.29.2.685-691.1980. View

Vann W, Schmidt M, JANN B, Jann K . The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin. Eur J Biochem. 1981; 116(2):359-64. DOI: 10.1111/j.1432-1033.1981.tb05343.x. View

Montreuil J, Strecker G, Dorland L, van Halbeek H, Veldink G, Vliegenthart F . Structures of fifteen oligosaccharides isolated from new-born meconium. Eur J Biochem. 1981; 117(2):291-300. DOI: 10.1111/j.1432-1033.1981.tb06336.x. View

Achtman M, Mercer A, Kusecek B, Pohl A, Heuzenroeder M, AARONSON W . Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun. 1983; 39(1):315-35. PMC: 347943. DOI: 10.1128/iai.39.1.315-335.1983. View

Pluschke G, Mercer A, Kusecek B, Pohl A, Achtman M . Induction of bacteremia in newborn rats by Escherichia coli K1 is correlated with only certain O (lipopolysaccharide) antigen types. Infect Immun. 1983; 39(2):599-608. PMC: 347994. DOI: 10.1128/iai.39.2.599-608.1983. View

10.

Schmidt M, JANN B, Jann K . Cell-wall lipopolysaccharide of the urinary-tract-infective Escherichia coli 04:K12:H-. Structure of the polysaccharide chain. Eur J Biochem. 1983; 137(1-2):163-71. DOI: 10.1111/j.1432-1033.1983.tb07810.x. View

11.

Kusecek B, Wloch H, Mercer A, Vaisanen V, Pluschke G, Korhonen T . Lipopolysaccharide, capsule, and fimbriae as virulence factors among O1, O7, O16, O18, or O75 and K1, K5, or K100 Escherichia coli. Infect Immun. 1984; 43(1):368-79. PMC: 263436. DOI: 10.1128/iai.43.1.368-379.1984. View

12.

Lvov V, Shashkov A, DMITRIEV B, Kochetkov N, JANN B, Jann K . Structural studies of the O-specific side chain of the lipopolysaccharide from Escherichia coli O:7. Carbohydr Res. 1984; 126(2):249-59. DOI: 10.1016/0008-6215(84)85382-3. View

13.

Strominger J, Park J, Thompson R . Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin. J Biol Chem. 1959; 234:3263-8. View

14.

Reske K, Jann K . The O8 antigen of Escherichia coli. Structure of the polysaccharide chain. Eur J Biochem. 1972; 31(2):320-8. DOI: 10.1111/j.1432-1033.1972.tb02536.x. View

15.

Tarcsay L, Wang C, Li S, Alaupovic P . Composition and structure of the O-specific side chain of endotoxin from Serratia marcescens 08. Biochemistry. 1973; 12(10):1948-55. DOI: 10.1021/bi00734a018. View

16.

Stellner K, Saito H, HAKOMORI S . Determination of aminosugar linkages in glycolipids by methylation. Aminosugar linkages of ceramide pentasaccharides of rabbit erythrocytes and of Forssman antigen. Arch Biochem Biophys. 1973; 155(2):464-72. DOI: 10.1016/0003-9861(73)90138-0. View

17.

Kaijser B . Immunology of Escherichia coli: K antigen and its relation to urinary-tract infection. J Infect Dis. 1973; 127(6):670-7. DOI: 10.1093/infdis/127.6.670. View

18.

JANN B, Reske K, Jann K . Heterogeneity of lipopolysaccharides. Analysis of polysaccharide chain lengths by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Eur J Biochem. 1975; 60(1):239-46. DOI: 10.1111/j.1432-1033.1975.tb20996.x. View

19.

De Bruyn A, Anteunis M . 1H-N.m.r. study of L-rhamnose, methyl alpha-L-rhamnopyranoside, and 4-o-beta-D-galactopranosyl-L-rhamnose in deuterium oxide. Carbohydr Res. 1976; 47(1):158-63. DOI: 10.1016/s0008-6215(00)83559-4. View