Human Fibronectin: Molecular Cloning Evidence for Two MRNA Species Differing by an Internal Segment Coding for a Structural Domain

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 Jan 1

PMID 6200322

Citations 70

Authors

A R Kornblihtt

F E Baralle

Affiliations

Soon will be listed here.

Abstract

Two different fibronectin (FN) mRNA species were detected in the human cell line Hs578T. One species, mRNA I, contains an additional 270 nucleotide long insert (ED) that encodes exactly one of the internally repeated structural domains of the protein. The 90 amino acid extra domain belongs to the so-called type III homology and it is located in the carboxy-terminal half of FN, in between the cell attachment and the heparin binding sites of the protein. The evidence of two mRNAs is provided by the isolation and characterisation of four independent cDNA clones from a library prepared with a synthetic oligonucleotide primer, and it was confirmed by S1 nuclease analysis of cDNA/mRNA hybrids. This kind of analysis also showed that in the human cell line, mRNA I is present at a lower level than mRNA II (the mRNA species without the ED), whilst in human liver, mRNA I is virtually undetectable. Since liver tissue has recently been reported to be the source of plasma FN, our results indicate that the presence of the ED insert could be a particular feature of cellular FN.

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References

Maxam A, Gilbert W . A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977; 74(2):560-4. PMC: 392330. DOI: 10.1073/pnas.74.2.560. View

Hackett A, Smith H, SPRINGER E, Owens R, Nelson-Rees W, Riggs J . Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines. J Natl Cancer Inst. 1977; 58(6):1795-806. DOI: 10.1093/jnci/58.6.1795. View

Hynes R, Destree A . Extensive disulfide bonding at the mammalian cell surface. Proc Natl Acad Sci U S A. 1977; 74(7):2855-9. PMC: 431319. DOI: 10.1073/pnas.74.7.2855. View

Berk A, Sharp P . Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977; 12(3):721-32. DOI: 10.1016/0092-8674(77)90272-0. View

Engvall E, Ruoslahti E, Miller E . Affinity of fibronectin to collagens of different genetic types and to fibrinogen. J Exp Med. 1978; 147(6):1584-95. PMC: 2184308. DOI: 10.1084/jem.147.6.1584. View

Yamada K, Kennedy D . Fibroblast cellular and plasma fibronectins are similar but not identical. J Cell Biol. 1979; 80(2):492-8. PMC: 2110339. DOI: 10.1083/jcb.80.2.492. View

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

Gergen J, STERN R, Wensink P . Filter replicas and permanent collections of recombinant DNA plasmids. Nucleic Acids Res. 1979; 7(8):2115-36. PMC: 342374. DOI: 10.1093/nar/7.8.2115. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

10.

Mosesson M, Amrani D . The structure and biologic activities of plasma fibronectin. Blood. 1980; 56(2):145-58. View

11.

Casadaban M, Cohen S . Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980; 138(2):179-207. DOI: 10.1016/0022-2836(80)90283-1. View

12.

Gait M, Singh M, SHEPPARD R, Edge M, Greene A, Heathcliffe G . Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates. Nucleic Acids Res. 1980; 8(5):1081-96. PMC: 323974. DOI: 10.1093/nar/8.5.1081. View

13.

Hagenbuchle O, Tosi M, Schibler U, BOVEY R, Wellauer P, Young R . Mouse liver and salivary gland alpha-amylase mRNAs differ only in 5' non-translated sequences. Nature. 1981; 289(5799):643-6. DOI: 10.1038/289643a0. View

14.

Atherton B, Hynes R . A difference between plasma and cellular fibronectins located with monoclonal antibodies. Cell. 1981; 25(1):133-41. DOI: 10.1016/0092-8674(81)90237-3. View

15.

Hayashi M, Yamada K . Differences in domain structures between plasma and cellular fibronectins. J Biol Chem. 1981; 256(21):11292-300. View

16.

Amara S, JONAS V, Rosenfeld M, Ong E, Evans R . Alternative RNA processing in calcitonin gene expression generates mRNAs encoding different polypeptide products. Nature. 1982; 298(5871):240-4. DOI: 10.1038/298240a0. View

17.

Pierschbacher M, Ruoslahti E, Sundelin J, Lind P, Peterson P . The cell attachment domain of fibronectin. Determination of the primary structure. J Biol Chem. 1982; 257(16):9593-7. View

18.

Shoulders C, Baralle F . Isolation of the human HDL apoprotein A1 gene. Nucleic Acids Res. 1982; 10(16):4873-82. PMC: 320834. DOI: 10.1093/nar/10.16.4873. View

19.

Hynes R, Yamada K . Fibronectins: multifunctional modular glycoproteins. J Cell Biol. 1982; 95(2 Pt 1):369-77. PMC: 2112946. DOI: 10.1083/jcb.95.2.369. View

20.

Skorstengaard K, Thogersen H, Petersen T, Magnusson S . Purification of twelve cyanogen bromide fragments from bovine plasma fibronectin and the amino acid sequence of eight of them. Overlap evidence aligning two plasmic fragments, internal homology in gelatin-binding region and phosphorylation site near.... Eur J Biochem. 1982; 128(2-3):605-23. DOI: 10.1111/j.1432-1033.1982.tb07007.x. View