Identification of the MT3 Molecule Using Two-dimensional Gel Electrophoresis and Alloantisera

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1983 Jan 1

PMID 6197369

Citations 4

Authors

M Suzuki

H Maeda

R Mukai

T Yabe

H Hamaguchi

Affiliations

Soon will be listed here.

Abstract

The MT3 antigen is defined serologically as a DR supertypic specificity and is strongly associated with DR4, DR7, and DRw9. To determine whether the MT3 molecule is distinct from the DR molecule, DR4 and MT3 antigens were immunoprecipitated from 125I-labeled plasma membrane glycoproteins of a DR4-homozygous, MT3-homozygous B lymphoid cell line, Wa, and compared by two-dimensional (2-D) gel electrophoresis. The precipitates with two different anti-DR4 alloantisera and with three different mouse antibodies against human Ia monomorphic determinants gave the same 2-D gel pattern consisting of one heavy chain with a molecular weight of 34 000 and a set of light chains with a molecular weight of 30 000, indicating that these polypeptides are the components of the DR4 molecule. On the other hand, all three anti-MT3 alloantisera used precipitated an identical set of anti-MT3 alloantisera specific light chains with a molecular weight of 30 000, and one heavy chain with a molecular weight of 34 000. The pI of the MT3 light chain was more acidic than that of the DR4 light chain. The amount of MT3 light chains was much smaller than that of DR4 light chains in unlabeled plasma membrane glycoproteins. Thus, we have demonstrated directly using 2-D gel electrophoresis and anti-MT3 alloantisera that the MT3 antigen is a new human Ia molecule distinct from DR4.

Citing Articles

Two-dimensional gel electrophoretic analysis of the MT3 and DR4 molecules from the different D-typed cells.

Suzuki M, Yabe T, Satake M, Juji T, Hamaguchi H J Exp Med. 1984; 160(3):751-8.

PMID: 6206187 PMC: 2187405. DOI: 10.1084/jem.160.3.751.

The MT3 specificity resides on a novel human class II antigen distinct from the HLA-DR antigen and DC-like antigen.

Yabe T, Suzuki M, Satake M, Juji T, Hamaguchi H Immunogenetics. 1984; 20(2):155-67.

PMID: 6205988 DOI: 10.1007/BF00364487.

Correlation between an HLA-DQ alpha length polymorphism of messenger RNA and serologically defined specificities (DQw1, DRw53, DR3+5).

Loiseau P, Lehn P, Dautry F, Lepage V, Colombani J, Cohen D Immunogenetics. 1986; 23(2):111-4.

PMID: 3007342 DOI: 10.1007/BF00377970.

Autoantibodies to T and B cell lines detected in serum samples from patients with systemic lupus erythematosus with lymphopenia and hypocomplementaemia.

Noguchi M, Iwamori M, Hirano T, Kobayashi S, Hashimoto H, Hirose S Ann Rheum Dis. 1992; 51(6):713-6.

PMID: 1616351 PMC: 1004731. DOI: 10.1136/ard.51.6.713.

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