Restriction and Modification in Bacillus Subtilis: Sequence Specificities of Restriction/modification Systems BsuM, BsuE, and BsuF

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Nov 1

PMID 6195145

Citations 23

Authors

S Jentsch

Affiliations

Soon will be listed here.

Abstract

The sequence specificities of three Bacillus subtilis restriction/modification systems were established: (i) BsuM (CTCGAG), an isoschizomer to XhoI; (ii) BsuE (CGCG), an isoschizomer to FnuDII; and (iii) BsuF (CCGG), an isoschizomer to MspI, HpaII. The BsuM modification enzyme methylates the 3' cytosine of the recognition sequence. The BsuF modification enzyme methylates the 5' cytosine of the sequence, rendering such sites resistant to MspI degradation and leaving the majority of sites sensitive to HpaII degradation.

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