» Articles » PMID: 6193333

Monoclonal Antibody to Keratin Filaments, Specific for Glandular Epithelia and Their Tumors. Use in Surgical Pathology

Overview
Journal Lab Invest
Specialty Pathology
Date 1983 Sep 1
PMID 6193333
Citations 69
Authors
Affiliations
Soon will be listed here.
Abstract

A monoclonal antibody (RGE 53) against keratin intermediate filaments was prepared by fusing myeloma cells with splenic lymphocytes from mice immunized with a HeLa cell cytoskeleton preparation and human callus keratins. The antibody, selected for fibrillar staining in HeLa cells and a negative reaction on human skin, was tested on frozen sections from normal and malignant human tissues. RGE 53 specifically recognizes glandular epithelial cells from female breast, digestive, respiratory, and urogenital tracts, endocrine and exocrine tissues, and mesothelial cells. No reaction is found in stratified squamous epithelia or nonepithelial tissues. Furthermore, RGE 53 can distinguish adenocarcinomas and mesotheliomas, which stain positively in the indirect immunofluorescence technique from squamous cell carcinomas and nonepithelial tumors, which are negative for this antibody. RGE 53 may, therefore, be a useful tool for differential diagnosis in surgical pathology, especially in those cases in which anaplastic carcinomas have to be characterized.

Citing Articles

Intermediate filament protein expression and mesoderm formation in the rabbit embryo : A double-labelling immunofluorescence study.

Viebahn C, Birgitte Lane E, Ramaekers F Rouxs Arch Dev Biol. 2017; 201(1):45-60.

PMID: 28305611 DOI: 10.1007/BF00188775.


Prognostic impact of serum CYFRA 21-1 in patients with advanced lung adenocarcinoma: a retrospective study.

Ono A, Takahashi T, Mori K, Akamatsu H, Shukuya T, Taira T BMC Cancer. 2013; 13:354.

PMID: 23879483 PMC: 3726411. DOI: 10.1186/1471-2407-13-354.


Intermediate filaments: a historical perspective.

Oshima R Exp Cell Res. 2007; 313(10):1981-94.

PMID: 17493611 PMC: 1950476. DOI: 10.1016/j.yexcr.2007.04.007.


Cytokeratin expression pattern in congenital and acquired pediatric cholesteatoma.

Olszewska E, Lautermann J, Koc C, Schwaab M, Dazert S, Hildmann H Eur Arch Otorhinolaryngol. 2005; 262(9):731-6.

PMID: 15754169 DOI: 10.1007/s00405-004-0875-0.


Up-regulation of neural stem cell markers suggests the occurrence of dedifferentiation in regenerating spinal cord.

Walder S, Zhang F, Ferretti P Dev Genes Evol. 2003; 213(12):625-30.

PMID: 14608505 DOI: 10.1007/s00427-003-0364-2.