Selective Phosphorylation of the IgE Receptor in Antigen-stimulated Rat Mast Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 May 1

PMID 6190168

Citations 6

Authors

B L Hempstead

C W Parker

A Kulczycki Jr

Affiliations

Soon will be listed here.

Abstract

Purified rat serosal mast cells were sensitized with mouse immunoglobulin E (IgE) anti-2,4-dinitrophenyl antibody, partially depleted of phosphate, labeled with [32P]orthophosphate, and stimulated with dinitrophenylated bovine serum albumin or control protein. After 15-120 seconds at 37 degrees C, the cells were extracted with nonionic detergent. IgE receptors were purified by repetitive affinity chromatography and were analyzed by NaDodSO4/polyacrylamide gel electrophoresis and radioautography. Antigenic stimulation of intact rat mast cells produced a rapid and marked increase in the phosphorylation of the surface-exposed alpha component of the IgE receptor. However, phosphorylation of the 33,000 Mr beta component of the IgE receptor was not altered significantly by antigen stimulation. This suggests that the selective increase in phosphorylation of the IgE receptor alpha component may be part of the physiologic mediator secretion process triggered by antigen.

Citing Articles

Lipid metabolism and the initiation and regulation of mediator release from mast cells.

Kennerly D Surv Immunol Res. 1984; 3(4):304-10.

PMID: 6239345 DOI: 10.1007/BF02919048.

Cells demonstrating Fc receptors for IgE.

Boltansky H, Kaliner M Surv Immunol Res. 1984; 3(2-3):99-102.

PMID: 6232681 DOI: 10.1007/BF02918772.

Identification of an IgE-binding protein by molecular cloning.

Liu F, Albrandt K, Mendel E, Kulczycki Jr A, Orida N Proc Natl Acad Sci U S A. 1985; 82(12):4100-4.

PMID: 3858867 PMC: 397942. DOI: 10.1073/pnas.82.12.4100.

Exocytosis induction in Paramecium tetraurelia cells by exogenous phosphoprotein phosphatase in vivo and in vitro: possible involvement of calcineurin in exocytotic membrane fusion.

Momayezi M, Lumpert C, Kersken H, Gras U, Plattner H, KRINKS M J Cell Biol. 1987; 105(1):181-9.

PMID: 3611184 PMC: 2114937. DOI: 10.1083/jcb.105.1.181.

An IgE-binding protein with a distinctive repetitive sequence and homology with an IgG receptor.

Albrandt K, Orida N, Liu F Proc Natl Acad Sci U S A. 1987; 84(19):6859-63.

PMID: 2958848 PMC: 299184. DOI: 10.1073/pnas.84.19.6859.

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