Expression of a Chemically Synthesized Human Alpha 1 Interferon Gene

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Jul 1

PMID 6181502

Citations 11

Authors

E De Maeyer

D Skup

K S Prasad

J De Maeyer-Guignard

B Williams

P Meacock

G Sharpe

D Pioli

J Hennam

W Schuch

K Atherton

Affiliations

Soon will be listed here.

Abstract

Cells of Escherichia coli containing a chemically synthesized human alpha 1 interferon (IFN-alpha 1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-alpha 1 [antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2'-5')oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-alpha 1 gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.

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References

Bennett P, Richmond M . Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Eschericha coli. J Bacteriol. 1976; 126(1):1-6. PMC: 233252. DOI: 10.1128/jb.126.1.1-6.1976. View

Marians K, Wu R, Stawinski J, Hozumi T, Narang S . Cloned synthetic lac operator DNA is biologically active. Nature. 1976; 263(5580):744-8. DOI: 10.1038/263744a0. View

Mantei N, Schwarzstein M, Streuli M, Panem S, Nagata S, Weissmann C . The nucleotide sequence of a cloned human leukocyte interferon cDNA. Gene. 1980; 10(1):1-10. DOI: 10.1016/0378-1119(80)90137-7. View

Windass J, Worsey M, Pioli E, Pioli D, Barth P, Atherton K . Improved conversion of methanol to single-cell protein by Methylophilus methylotrophus. Nature. 1980; 287(5781):396-401. DOI: 10.1038/287396a0. View

Allen G, Fantes K . A family of structural genes for human lymphoblastoid (leukocyte-type) interferon. Nature. 1980; 287(5781):408-11. DOI: 10.1038/287408a0. View

Goeddel D, Leung D, Dull T, Gross M, Lawn R, McCandliss R . The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature. 1981; 290(5801):20-6. DOI: 10.1038/290020a0. View

Keshet E, Rosner A, Bernstein Y, Gorecki M, Aviv H . Cloning of bovine growth hormone gene and its expression in bacteria. Nucleic Acids Res. 1981; 9(1):19-30. PMC: 326665. DOI: 10.1093/nar/9.1.19. View

Stewart 2nd W, Sarkar F, Taira H, Hall A, Nagata S, Weissmann C . Comparisons of several biological and physicochemical properties of human leukocyte interferons produced my human leukocytes and by E. coli. Gene. 1980; 11(3-4):181-6. DOI: 10.1016/0378-1119(80)90058-x. View

Yelverton E, Leung D, Weck P, Gray P, Goeddel D . Bacterial synthesis of a novel human leukocyte interferon. Nucleic Acids Res. 1981; 9(3):731-41. PMC: 327233. DOI: 10.1093/nar/9.3.731. View

10.

Streuli M, Hall A, Boll W, Stewart 2nd W, Nagata S, Weissmann C . Target cell specificity of two species of human interferon-alpha produced in Escherichia coli and of hybrid molecules derived from them. Proc Natl Acad Sci U S A. 1981; 78(5):2848-52. PMC: 319455. DOI: 10.1073/pnas.78.5.2848. View

11.

Edge M, Green A, Heathcliffe G, Meacock P, Schuch W, Scanlon D . Total synthesis of a human leukocyte interferon gene. Nature. 1981; 292(5825):756-62. DOI: 10.1038/292756a0. View

12.

Hitzeman R, HAGIE F, Levine H, Goeddel D, Ammerer G, Hall B . Expression of a human gene for interferon in yeast. Nature. 1981; 293(5835):717-22. DOI: 10.1038/293717a0. View

13.

Hardy K, Stahl S, Kupper H . Production in B. subtilis of hepatitis B core antigen and a major antigen of foot and mouth disease virus. Nature. 1981; 293(5832):481-3. DOI: 10.1038/293481a0. View

14.

Hennam J, Cunningham A, Sharpe G, Atherton K . Expression of eukaryotic coding sequences in Methylophilus methylotrophus. Nature. 1982; 297(5861):80-2. DOI: 10.1038/297080a0. View

15.

Isaacs A, Lindenmann J, Valentine R . Virus interference. II. Some properties of interferon. Proc R Soc Lond B Biol Sci. 1957; 147(927):268-73. DOI: 10.1098/rspb.1957.0049. View