Biological Functions of T Cell Lines with Specificity for the Intracellular Bacterium Listeria Monocytogenes in Vitro and in Vivo

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1982 Jun 1

PMID 6176667

Citations 64

Authors

S H Kaufmann

H Hahn

Affiliations

Soon will be listed here.

Abstract

Peritoneal exudate T lymphocytes from mice immunized with live Listeria monocytogenes were cloned in double-layer soft agar containing heat-killed L. monocytogenes (lower layer) and syngeneic accessory cells (upper layer). Colony-derived T cells were propagated in vitro in the presence of listerial antigen, syngeneic accessory cells, and T cell growth factor. In vitro proliferation, interleukin secretion, and bystander help for B cells of six such T cell lines and several sublines derived from them were found to be antigen dependent and restricted by the H-2IA locus of the major histocompatibility complex. In vivo, these T cell lines conferred delayed-type hypersensitivity to listerial antigen and protection to live L. monocytogenes. It is concluded that different biological functions of acquired antibacterial immunity can be mediated by a single T cell population.

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