Regulation of Actin Polymerizaton in Rat Islets of Langerhans

Overview

Journal Biochem J

Specialty Biochemistry

Date 1980 Oct 15

PMID 6171248

Citations 8

Authors

S L Howell

M Tyhurst

Affiliations

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Abstract

A DNAase-inhibition assay was used to determine the proportions of globular (G-) and filamentous (F-) actin in islets of Langerhans after incubation in various conditions, or after subcellular fractionation. Stimulation of insulin secretion resulted in an ATP-dependent increase in the proportion of F-actin present; fractionation showed 80-90% of the actin to be present in the final supernatant.

Citing Articles

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Glucose-induced ERM protein activation and translocation regulates insulin secretion.

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Effect of disruption of actin filaments by Clostridium botulinum C2 toxin on insulin secretion in HIT-T15 cells and pancreatic islets.

Li G, Rungger-Brandle E, Just I, Jonas J, Aktories K, Wollheim C Mol Biol Cell. 1994; 5(11):1199-213.

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Activation of alpha-2-adrenoceptors results in an increase in F-actin formation in HIT-T15 pancreatic B-cells.

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References

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